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纳索尼亚维特里彭尼斯的 Rnai 和克里斯普基因编辑的普帕尔和成人注射
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纳索尼亚维特里彭尼斯的 Rnai 和克里斯普基因编辑的普帕尔和成人注射

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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在这里，我们将 纳索尼亚维特里彭尼斯 高效注射 pupal 和成人注射的方法描述为胚胎微注射的可访问替代品，通过 RNA 干扰 （RNAi） 或通过 CRISPR/Cas9 基因组编辑进行基因敲除，实现感兴趣的基因的功能分析。

Tags

生物学， 第 166 期， 纳索尼亚维特里彭尼斯， CRISPR /Cas9， 雷莫特， BAPC 辅助 CRISPR， 成人注射， RNAi
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