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Pupal och vuxna injektioner för RNAi och CRISPR Genredigering i Nasonia vitripennis
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Pupal och vuxna injektioner för RNAi och CRISPR Genredigering i Nasonia vitripennis

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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Här beskriver vi metoder för effektiva poppal- och vuxeninjektioner i Nasonia vitripennis som tillgängliga alternativ till embryomikroinjektion, vilket möjliggör funktionell analys av gener av intresse med antingen RNA-ljuddämpande via RNA-interferens (RNAi) eller gen knockout via CRISPR / Cas9 genomredigering.

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Biologi Nummer 166 Nasonia vitripennis,CRISPR/Cas9 ReMOT BAPC-assisterad CRISPR Vuxeninjektioner RNAi
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