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Bioengineering

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JoVE Journal Bioengineering
En kulturmetode for å opprettholde quiescent humane hematopoietiske stamceller
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En kulturmetode for å opprettholde quiescent humane hematopoietiske stamceller

Article DOI: 10.3791/61938-v • 07:14 min • May 17th, 2021
May 17th, 2021 •
1, 1
1Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine

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Denne protokollen gjør det mulig å opprettholde quiescent human hematopoietiske stamceller in vitro ved å etterligne benmargsmikromiljøet ved hjelp av rikelig fettsyre, kolesterol, lavere konsentrasjoner av cytokiner og hypoksi.

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Bioingeniør Utgave 171 Hematopoietiske stamceller passivisert fettsyre kolesterol hypoksi benmargsmikromiljø
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