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Journal / Biochemistry / 使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量…
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JoVE Journal Biochemistry
使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量
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使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量

Article DOI: 10.3791/62608-v • 11:38 min • September 14th, 2021
September 14th, 2021 •
1,2, 1, 1
1Institute for Chemistry and Biochemistry, Freie Universität Berlin, 2International Max-Planck Research School for Biology and Computation

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在这里，我们建立了一个方案，使用暴露于病理和生理流体剪切应激条件下的内皮细胞中的接近连接测定（PLA）同时可视化和分析多个SMAD复合物。

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生物化学，第175期，流体剪切应激，接近连接测定，内皮细胞，SMADs，BMP，TGFβ，动脉粥样硬化，流动
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