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Journal / Biochemistry / 近接ライゲーションアッセイを用いた異流体せん断応力条件下でのTGFβ/BMP/SMADシグナル伝達の…
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JoVE Journal Biochemistry
近接ライゲーションアッセイを用いた異流体せん断応力条件下でのTGFβ/BMP/SMADシグナル伝達の可視化と定量化
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近接ライゲーションアッセイを用いた異流体せん断応力条件下でのTGFβ/BMP/SMADシグナル伝達の可視化と定量化

Article DOI: 10.3791/62608-v • 11:38 min • September 14th, 2021
September 14th, 2021 •
1,2, 1, 1
1Institute for Chemistry and Biochemistry, Freie Universität Berlin, 2International Max-Planck Research School for Biology and Computation

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ここでは、病理的および生理的な流体せん断ストレス条件に曝露した内皮細胞における近接ライゲーションアッセイ(PLA)を用いて、複数のSMAD複合体を同時に可視化・解析するプロトコルを確立する。

Tags

生化学、問題175、流体せん断応力、近接ライゲーションアッセイ、内皮細胞、SMA、BMP、TGFβ、アテローム性動脈硬化症、流れ
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