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Isolering og karakterisering af cyanobakterielle ekstracellulære vesikler
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Isolering og karakterisering af cyanobakterielle ekstracellulære vesikler

Article DOI: 10.3791/63481-v • 08:44 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 3,4,5, 3,4, 3,4,6, 3,4,6
1Department of Biological Sciences, Wellesley College, 2Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario, Universidad de Córdoba, 3i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 5MCbiology Doctoral Program, ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 6Departamento de Biologia, Faculdade de Ciências, Universidade do Porto
* These authors contributed equally

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Den nuværende protokol indeholder detaljerede beskrivelser af isolering, koncentration og karakterisering af ekstracellulære vesikler fra cyanobakterielle kulturer. Tilgange til rensning af vesikler fra kulturer i forskellige skalaer, afvejninger mellem metoder og overvejelser om at arbejde med feltprøver diskuteres også.

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