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Isolation and Characterization of Cyanobacterial Extracellular Vesicles
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Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Article DOI: 10.3791/63481-v • 08:44 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 3,4,5, 3,4, 3,4,6, 3,4,6
1Department of Biological Sciences, Wellesley College, 2Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario, Universidad de Córdoba, 3i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 5MCbiology Doctoral Program, ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 6Departamento de Biologia, Faculdade de Ciências, Universidade do Porto
* These authors contributed equally

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The present protocol providesdetailed descriptions for isolation, concentration,and characterization of extracellular vesicles from cyanobacterial cultures. Approaches for purifying vesicles from cultures at different scales, trade-offs among methodologies, and considerations for working with field samples are also discussed.

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Isolation Characterization Cyanobacterial Extracellular Vesicles Purification Culture Trade Offs Methodologies Field Samples Sampling Analysis Concentration Ultrafiltration Centrifugal Concentrators Type 1 Ultrapure Grade Water Supernatant Sample Final Volume Tangential Flow Filtration Apparatus Peristaltic Pump Retentate Line Sanitize Back Pressure Levels
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