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从微解剖小鼠脉络丛中分离和表征免疫细胞

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

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从微解剖小鼠脉络丛中分离和表征免疫细胞

DOI:

10.3791/63487-v

•

09:55 min

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February 03, 2022

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Amaia Dominguez-Belloso, Sandrine Schmutz, Sophie Novault, Laetitia Travier*¹, Aleksandra Deczkowska*¹

¹Brain-Immune Communication Lab,Institut Pasteur, ²Cytometry platform, CB UTechS,Institut Pasteur

Capítulos

00:05Introduction
00:42Dissection of the Choroid Plexus from the Brain
02:47Preparation of Samples for Flow Cytometry Analysis
03:52Choroid Plexus Myeloid Cells Gating
06:46Choroid Plexus T Cells Gating
07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
09:24Conclusion

Summary

Tadução automática

本研究使用流式细胞术和两种不同的门控策略对分离的灌注小鼠脑脉络丛;该协议确定了填充该大脑结构的主要免疫细胞亚群。

Tags

Immune Cells Choroid Plexuses Mouse Model Brain Regulation Cell Dissection Cell Isolation Cell Characterization Collagenase IV Lateral Ventricle Third Ventricle Fourth Ventricle Cell Dissociation MACS Buffer Cell Centrifugation Cell Washing

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