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JoVE Journal Cancer Research
Visualisierung von Proteinen zur Reparatur von DNA-Schäden in patienteneigenen Ovarialkarzinom-Organoiden mittels Immunfluoreszenz-Assays
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Visualisierung von Proteinen zur Reparatur von DNA-Schäden in patienteneigenen Ovarialkarzinom-Organoiden mittels Immunfluoreszenz-Assays

Article DOI: 10.3791/64881-v • 04:07 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1, 1, 1,2, 1, 1
1Washington University in St. Louis, 2University of California San Francisco

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Das vorliegende Protokoll beschreibt Methoden zur Bewertung von DNA-Schadensreparaturproteinen in von Patientinnen stammenden Ovarialkarzinom-Organoiden. Dazu gehören umfassende Plattierungs- und Färbemethoden sowie detaillierte, objektive Quantifizierungsverfahren.

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Krebsforschung Heft 192
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