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Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Article DOI: 10.3791/64917-v • 08:19 min • February 10th, 2023
February 10th, 2023 •
1,2, 1, 3, 2, 4, 1, 1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

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Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.

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Pig Intestinal Organoids Cryopreserved Epithelial Crypts Cell Monolayers Veterinary Research Biomedical Research Nutrient Transport Barrier Function Host-microbe Interactions Jejunum Duodenum Ileum Colon Thawing Centrifuge Extracellular Matrix (ECM) Seeding Wells Polymerization Culture Medium
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