Metabolic Product Quantification
Metabolic Product Quantification - Related Videos
10:01
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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Cited by 39 •
2012
The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
10:20
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
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Cited by 14 •
2015
Integration of microalgal cultivation with industrial flue gas will ultimately introduce heavy metals and other inorganic compounds into the growth media. This study presents a procedure used to determine the end fate and impact of heavy metals and inorganic contaminants on the growth of Nannochloropsis salina grown in photobioreactors.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
FAQs
A calibration curve links measured signal intensity to concentration by analyzing known standards under the same measurement conditions as the samples. The unknown sample signal is then compared with this reference relationship to estimate its metabolite concentration. This step makes results quantitative rather than merely indicating that a metabolic product is present.
Sample preparation must preserve the analytes before analysis, because changes during handling can alter the measured result. In metabolic studies, reliable preparation supports accurate comparisons among cells, tissues, or reaction mixtures. It therefore affects how confidently investigators can evaluate reaction rates, nutrient utilization, product yield, or responses to experimental changes.
Repeated quantitative measurements can show whether a metabolite is being produced or consumed and how its amount changes during a biochemical process. Those changes provide evidence about reaction rates and pathway regulation, rather than only confirming detection. Comparing results across conditions can also reveal effects associated with genetic or environmental changes.
A typical workflow begins by collecting and preparing the biological or biochemical sample so analytes remain preserved. The sample is then analyzed by spectrophotometry, chromatography, or mass spectrometry. Finally, signal intensity is compared with known standards or a calibration curve, producing concentration values that can be interpreted in relation to the experimental system.
These methods represent alternative analytical approaches for measuring metabolic products, and the appropriate choice depends on the analytes and the study objective. The overview identifies all three as established options, while emphasizing that quantitative interpretation requires comparison with standards or calibration curves. Their results can support enzyme studies, cellular metabolism research, or bioprocess investigations.
In biochemistry, these measurements help connect observed metabolite levels with enzyme activity, pathway regulation, nutrient use, and product yield. Researchers can apply the results to microbial and cellular metabolism, disease investigation, bioprocess optimization, and assessment of metabolic responses to genetic or environmental changes. The quantitative outcomes support comparisons among experimental conditions.