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Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

DOI:
10.3791/61703-v

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07:28 min

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October 29, 2020

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, , ,
1Helmholtz Institute for Pharmaceutical Research Saarland, Department of Microbial Natural Products, Helmholtz Centre for Infection Research and Department of Pharmacy,Saarland University, 2Partner Site Hannover-Braunschweig,German Centre for Infection Research (DZIF)

Chapters

  • 00:04Introduction
  • 00:42Sample Preparation
  • 01:20Insert Preparation and Loading
  • 02:10Sample Analysis
  • 03:37Data Analysis
  • 05:07Results: Representative Antibiotic Effects on A. baumannii DSM-30008 Growth and Metabolism
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.

Tags

Keywords: Metabolic ProfilingBactericidalBacteriostaticNatural ProductsIsothermal MicrocalorimetryAntibacterial CompoundsMode Of ActionMicrobiological TechniquesSpectrophotometerOptical DensityColony Forming UnitsAntibioticVortexingPlastic InsertsTitanium VialsSample Insertion ArmData Analysis

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