Rt-lamp Assay
Rt-lamp Assay - Related Videos
07:47
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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Cited by 5 •
2020
We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.
08:03
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
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Cited by 8 •
2018
This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.
06:57
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
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Cited by 10 •
2015
Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.
12:32
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
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Cited by 13 •
2016
Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.
12:04
RNA Analysis of Environmental Samples Using RT-PCR
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Reverse transcription-polymerase chain reaction (RT-PCR) involves the same process as conventional PCR — cycling temperature to amplify nucleic acids. However, while conventional PCR only amplifies deoxyribonucleic acids (DNA), RT-PCR enables the amplification of ribonucleic acids (RNA) through the formation of complementary DNA (cDNA). This enables RNA-based organisms...
FAQs
The primer set recognizes multiple regions of the RNA-derived target, allowing amplification to begin at several positions. As strand-displacing synthesis proceeds, the newly formed DNA adopts loop-shaped structures. These loops provide additional sites for primer binding and support autocycling, helping the reaction generate detectable amplification rapidly while remaining at a constant temperature.
Reverse transcriptase first converts the RNA target into complementary DNA, creating a DNA template for subsequent amplification. The strand-displacing DNA polymerase then extends primers while displacing existing DNA strands rather than requiring thermal separation. Together, these enzyme activities connect RNA recognition with continuous DNA synthesis under isothermal conditions.
Amplification can be followed through color change, turbidity, or fluorescence, so the assay does not depend on a single detection format. These observable signals provide a way to determine whether the reaction has produced amplified material. The choice of readout can therefore be matched to the available instrumentation and the desired accessibility of the analysis.
The main practical distinction is temperature control: conventional PCR relies on repeated thermal cycling, whereas RT-LAMP maintains a constant reaction temperature. Because it can use color, turbidity, or fluorescence for readout and requires minimal instrumentation, RT-LAMP can support analyses in settings where thermal-cycling equipment is unavailable or impractical.
The workflow begins with an RNA target and uses reverse transcriptase to produce complementary DNA. Multiple primers and strand-displacing polymerase then drive amplification through loop-forming, autocycling DNA structures at a constant temperature. The reaction is subsequently assessed by color, turbidity, or fluorescence, yielding an observable result without conventional thermal cycling.
RT-LAMP is useful when researchers need to detect RNA-associated targets with accessible analysis. Supported applications include identifying RNA viruses, examining gene expression targets, and detecting other analytes. Its constant-temperature operation and minimal instrumentation are particularly relevant to molecular diagnostic work in resource-limited settings, where conventional thermal-cycling platforms may be less accessible.