3d Cardiac Tissue
3d Cardiac Tissue - Related Videos
04:40
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Cited by 55 •
2017
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
10:42
Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
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Cited by 4 •
2021
The goal of this protocol is to explain and demonstrate the development of a three-dimensional (3D) microfluidic model of highly aligned human cardiac tissue, composed of stem cell-derived cardiomyocytes co-cultured with cardiac fibroblasts (CFs) within a biomimetic, collagen-based hydrogel, for applications in cardiac tissue engineering, drug screening, and disease modeling.
10:09
Capillary Force Lithography for Cardiac Tissue Engineering
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Cited by 27 •
2014
In this protocol, we demonstrate the fabrication of biomimetic cardiac cell culture substrata made from two distinct polymeric materials using capillary force lithography. The described methods provide a scalable, cost-effective technique to engineer the structure and function of macroscopic cardiac tissues for in vitro and in vivo applications.
11:51
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Cited by 16 •
2016
This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.
FAQs
Electrical coupling helps align the activity of neighboring cardiomyocytes, allowing contractions to occur in a coordinated manner rather than as isolated cellular events. In a 3D cardiac tissue model, this interaction can be examined alongside cell-cell signaling and extracellular matrix contacts. The resulting functional behavior helps bioengineers evaluate how tissue organization contributes to heart-like performance.
Supporting cells and biomaterials do more than provide physical bulk. Together with cardiomyocytes, they contribute to the cellular and structural environment in which signaling, matrix interactions, and electrical coordination occur. Changing this composition or organization can therefore alter tissue behavior, making these components important variables when investigating maturation, disease-related changes, or responses to interventions.
Spheroids, scaffolds, and larger tissue constructs offer different ways to organize cardiac cells and biomaterials in three dimensions. Their distinct architectures provide alternative settings for examining cell-cell signaling, extracellular matrix interactions, electrical coupling, and contraction. Comparing these formats can help researchers determine how the physical arrangement of an engineered model influences the cardiac functions it reproduces.
Tissue architecture affects how cardiac cells interact with one another and with the extracellular matrix. Those interactions influence the coordination of electrical activity and contraction, so a model's three-dimensional organization can shape its functional behavior. Studying this relationship allows bioengineers to connect structural features with heart function and to assess whether an engineered tissue captures relevant myocardial characteristics.
Researchers can arrange cardiomyocytes, supporting cells, and biomaterials into spheroids, scaffolds, or broader tissue constructs. This organization creates controlled three-dimensional environments in which signaling, matrix interactions, and electrical coupling can be studied together. Selecting an appropriate format helps align the model with the feature under investigation, such as tissue architecture, coordinated contraction, or cellular responses.
These models can support investigations of cardiac development, disease mechanisms, and responses to drugs or electrical stimulation. Because the cells are organized within a three-dimensional engineered environment, researchers can examine how tissue structure and coordinated function relate to these processes. The approach therefore provides a controlled platform for connecting cellular behavior with broader cardiac outcomes.
Within bioengineering, these engineered tissues provide controlled systems for studying how myocardial architecture influences function. Their use may improve preclinical testing by enabling drug responses and disease-related behavior to be examined in an organized tissue setting. The same research can also guide regenerative strategies by revealing how engineered structure, cellular interactions, and cardiac performance are connected.