Liquid Handling
Liquid Handling - Related Videos
08:24
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
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Cited by 5 •
2008
The Ugi reaction has proved to be a convenient way to quickly create diverse libraries of compounds. It involves the reaction of an amine, an aldehyde, a carboxylic acid and an isonitrile typically in methanol at room temperature. In this video, we utilize a 48-slot Mettler-Toledo MiniBlock equipped with filtration tubes and a Mettler-Toledo MiniMapper automated liquid handler was used to deliver the reagents and solvent. The parameters of interest were the concentration, the solvent...
08:00
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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Cited by 23 •
2015
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
11:22
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
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Cited by 19 •
2017
Here, an automated workflow to perform modular DNA "device" assembly using a modular cloning DNA assembly method on liquid-handling robots is presented. The protocol uses an intuitive software tool for generating liquid handler picklists for combinatorial DNA device library generation, which we demonstrate using two liquid handling platforms.
10:47
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
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Cited by 13 •
2013
TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.
03:51
Handling Chemical Spills
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2023
Source: Robert M. Rioux & Taslima Zaman, Pennsylvania State University, University Park, PA Since chemicals are commonly used for laboratory research purposes, it is extremely important to be adequately prepared to handle chemical spills or accidental release of hazardous chemicals, which can happen at any time. No matter how minor a spill may be, the inability to respond in an emergency situation could severely endanger public health or the environment. All chemical spills must be properly...
FAQs
Viscosity, surface tension, and temperature can alter how a liquid behaves during aspiration, transfer, and dispensing. These factors influence whether the intended volume moves consistently and whether liquid remains on the tool or container surface. Accounting for them helps researchers maintain defined volumes, especially when preparing biological samples, reagents, or serial dilutions.
Tip selection contributes to consistent volume transfer and helps limit contamination during biological workflows. An unsuitable tip can interact differently with liquids affected by viscosity or surface tension, increasing the chance of incomplete transfer or retained liquid. Choosing the tip with the liquid properties and task in mind supports reproducible sample preparation and assay setup.
Automated liquid handling uses robotic platforms to perform transfers and additions with greater throughput and standardization, while manual work relies on an operator using tools such as pipettes, syringes, or dispensers. Automation is particularly useful when many samples require repeated operations, whereas manual handling remains relevant for smaller or less standardized workflows.
A reliable workflow begins by selecting an appropriate transfer tool and considering the liquid's viscosity, surface tension, and temperature. The operator then measures or moves the intended volume, mixes when required, and dispenses it into the receiving vessel. Attention to tip selection, bubbles, and contamination throughout the sequence helps preserve accuracy and reproducibility.
Careful transfers support sample preparation, serial dilution, reagent addition, cell culture, nucleic acid extraction, and assay setup. Each procedure depends on moving the intended liquids into the correct containers and combinations. Errors in volume, mixing, contamination control, or bubble formation can compromise the consistency of downstream biological results, making technique central to experimental reliability.
Standardized liquid handling supports diagnostics, drug discovery, and genomics by making repeated sample and reagent operations more consistent. Robotic platforms can increase throughput when experiments involve many transfers, while careful manual technique supports smaller-scale work. Across these settings, reducing contamination, bubbles, and volume error helps produce more reproducible biological measurements and preparations.