Simulated Intestinal Fluid
Simulated Intestinal Fluid - Related Videos
12:39
Computational Fluid Dynamics Simulations of Blood Flow in a Cerebral Aneurysm
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2023
Source: Joseph C. Muskat, Vitaliy L. Rayz, and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana The objective of this video is to describe recent advancements of computational fluid dynamic (CFD) simulations based on patient- or animal-specific vasculature. Here, subject-based vessel segmentations were created, and, using a combination of open-source and commercial tools, a high-resolution numerical solution was determined within a flow model.
02:54
Exposing a Synthetic Oral Bacterial Community to Simulated Phases of Digestion
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2025
Source: Arroyo, M. C., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018)This video demonstrates the viability assessment of a synthetic oral microbial community during simulated oral, gastric, and intestinal digestion. Bacterial survival is quantified after each phase, revealing a progressive decline due to digestive stress.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
06:37
Analyzing Melts and Fluids from Ab Initio Molecular Dynamics Simulations with the UMD Package
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Cited by 2 •
2021
Melts and fluids are ubiquitous vectors of mass transport in natural systems. We have developed an open-source package to analyze ab initio molecular-dynamics simulations of such systems. We compute structural (bonding, clusterization, chemical speciation), transport (diffusion, viscosity) and thermodynamic properties (vibrational spectrum).
10:20
In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
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Cited by 2 •
2022
A pendant drop surface film balance implemented with a multi-subphase exchange, nicknamed the OCTOPUS, allows for mimicking digestive conditions by the sequential subphase exchange of the original bulk solution with simulated gastrointestinal fluids. The simulated in vitro digestion is monitored by recording in situ the interfacial tension of the digested interfacial layer.
FAQs
Defined pH and aqueous buffering establish the chemical environment in which a substance is tested after stomach passage. These conditions influence whether a material dissolves, remains stable, or participates in digestion and solubilization processes. Holding them constant allows researchers to compare intestinal behavior across compounds, formulations, or treatments without changes caused by uncontrolled chemical conditions.
Bile-related components and digestive enzymes represent important chemical influences encountered in the small intestine. Together, they help investigators examine solubilization and digestion rather than measuring dissolution in a simple buffer alone. Including these components can reveal how a nutrient, drug, or formulation behaves when intestinal factors affect its chemical state and release.
A standardized intestinal model reduces experimental variability by using defined component concentrations and pH values. Researchers can therefore compare materials or treatments under the same conditions and identify differences in dissolution, stability, digestion, or solubilization. The approach complements, rather than replaces, biological studies by providing controlled evidence before more variable in vivo evaluation.
A typical workflow establishes the prepared medium with its selected aqueous buffer, bile-related components, digestive enzymes, concentrations, and pH. The substance is then evaluated under those intestinal conditions for behaviors such as dissolution, digestion, solubilization, or stability. Results can be compared across formulations or treatments because the surrounding chemical environment remains defined and consistent.
Pharmaceutical researchers can use Simulated Intestinal Fluid to investigate how an oral formulation releases and behaves after leaving the stomach. Measurements of dissolution, compound stability, and solubilization help compare formulation designs and support oral delivery studies. This information can guide evaluation of whether different materials or treatments produce distinct intestinal performance under standardized laboratory conditions.
Studies in this medium provide a controlled way to examine intestinal behavior before assessing potential absorption in broader biological research. For nutrients and drugs, researchers can investigate release, digestion, stability, and solubilization under defined conditions. The resulting comparisons help connect a substance’s chemical behavior in the small-intestinal environment with questions about oral delivery and absorption potential.