Intestinal Perfusion Studies
Intestinal Perfusion Studies - Related Videos
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
07:00
Using Caco-2 Cells to Study Lipid Transport by the Intestine
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Cited by 11 •
2015
Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
07:00
Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine
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Cited by 13 •
2019
Here, we present a powerful and physiological model to study the molecular mechanisms underlying gut hormone secretion and intestinal absorption — the isolated perfused rat small intestine.
FAQs
They examine circulation at more than one level. Measurements of blood movement through mesenteric arteries can reveal reduced inflow or vascular obstruction, while assessment of the intestinal microcirculation addresses how effectively blood reaches bowel tissues. Considering both levels helps clinicians relate visible vascular abnormalities to the local oxygen supply required to preserve intestinal viability.
Blood flow alone does not fully describe bowel health because intestinal tissue also depends on adequate oxygen delivery. Perfusion studies therefore may incorporate physiologic indicators that reflect altered tissue oxygenation. Linking these findings with vascular measurements helps identify circulatory failure that could threaten bowel viability, particularly when reduced flow has not yet produced irreversible tissue injury.
The findings may indicate reduced mesenteric blood flow, vascular obstruction, or changes in tissue oxygenation. These abnormalities provide different clues about how circulation is failing rather than simply labeling the bowel as well or poorly perfused. Interpreting them together helps clinicians assess whether gastrointestinal disease is associated with impaired vascular function and possible progression toward tissue injury.
Clinical evaluation commonly uses imaging or physiologic indicators to examine blood movement and tissue oxygenation. The selected assessment can address mesenteric arteries, the intestinal microcirculation, or both, depending on the clinical question. Results are then considered in relation to bowel viability and the suspected cause of circulatory impairment, including obstruction or postoperative hypoperfusion.
They are particularly relevant when clinicians suspect acute mesenteric ischemia, intestinal infarction, or inadequate perfusion after surgery. In these settings, the studies help monitor whether circulation is deteriorating or improving and support decisions that may need to be made before inadequate blood supply causes irreversible bowel injury. Their value lies in connecting perfusion findings with tissue viability.
By showing how vascular function affects the bowel, these investigations add physiologic context to the clinical assessment of gastrointestinal disease. Evidence of impaired flow or altered oxygenation can support recognition of circulatory failure and help guide urgent treatment decisions. Repeated assessment may also help track postoperative hypoperfusion or evolving ischemic injury over time.