Non-human Primate Islets
Non-human Primate Islets - Related Videos
07:12
Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption
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Cited by 1 •
2019
This protocol demonstrates the accurate and reproducible measurement of oxygen consumption in non-human primate pancreatic islets. The islet loading techniques and coating of the microplate provide a framework for efficient measurement of respiration in other types of cultured spheroids.
16:00
Behavioral Assessment of Manual Dexterity in Non-Human Primates
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Cited by 45 •
2011
As manual dexterity is a prerogative mainly of primates, behavioral tasks have been developed in macaque monkeys. Four reach and grasp prehension tasks, measuring hand manipulation ability and force, allow to establish functional recovery after a lesion of the central nervous system and to test the effect of a treatment.
09:21
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
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Cited by 60 •
2010
We demonstrate the use of the gene gun to introduce fluorescent dyes, such as DiI, into neurons in brain slices from rodents and non-human primates of different ages. In this particular case, we use adult mice (3-6 months old) and adult cynomologus monkeys (9-15 years old). This technique, originally described by the laboratory of Dr. Lichtman (Gan et al., 2000), is well suited for the study of dendritic branching and dendritic spine morphology and can be combined with traditional...
03:56
Performing Immunohistochemistry on a Non-Human Primate Brain Tissue
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2025
Source: Eid, L. & Parent, M. Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy. J. Vis. Exp. (2017).This video demonstrates the preparation of aldehyde-fixed non-human primate brain tissue using pre-embedding immunohistochemistry for electron microscopy. It outlines the steps for immunolabeling a specific neuronal protein, followed by a peroxidase reaction that produces a colored precipitate, enabling visualization of the target...
09:09
Dissecting the Non-human Primate Brain in Stereotaxic Space
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Cited by 14 •
2009
The non-human primate is an important translational species for our understanding of the normal processing of the brain. The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans.
FAQs
Cellular organization provides a way to connect islet structure with endocrine function during development. Researchers examine where beta cells and other endocrine cells are positioned, how that arrangement changes over time, and whether organization accompanies maturation. This approach can reveal developmental patterns that affect coordinated hormone secretion, rather than treating each endocrine cell type as an isolated unit.
Comparing developmental stages shows how immature endocrine tissue progresses toward a more mature islet state. Investigators can track formation, cellular organization, maturation, and hormone secretion as sequential features, then ask which changes are conserved across primates and which are primate-specific. That temporal perspective is especially relevant when evaluating developmental models of pancreatic disease or engineered beta cells.
Rising glucose engages beta cells as the insulin-secreting component, while other endocrine cells release hormones that coordinate metabolic control. Studying these cell populations together helps researchers assess both the beta-cell response and the broader endocrine context in which it occurs. The resulting view is useful for interpreting how developmental changes may influence overall islet function.
Non-human primate islets complement rodent models by providing biological context that is closer to human pancreatic development, while still allowing researchers to examine developmental organization and endocrine function experimentally. Their comparison can expose features shared across species as well as primate-specific characteristics. This distinction helps investigators judge how confidently findings from simpler animal models may translate to human biology.
Studies commonly compare islets from different developmental stages and evaluate three linked features: formation, cellular organization, and maturation. Researchers also assess hormone secretion, including the insulin response associated with beta cells, to connect structural changes with function. Combining these observations produces a developmental profile that can guide interpretation of disease models and stem cell-derived beta-cell systems.
These islets provide a reference for testing whether stem cell-derived beta cells resemble developing or mature primate tissue. Researchers can compare cellular organization, maturation, and hormone secretion with corresponding features in primate islets. Such benchmarking identifies biological gaps in engineered cells and supplies developmental context for improving models intended for diabetes research, transplantation, or regenerative approaches.