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JoVE Journal
Neuroscience

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DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
 

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Article doi: 10.3791/2081
July 6th, 2010

Summary July 6th, 2010

We demonstrate the use of the gene gun to introduce fluorescent dyes, such as DiI, into neurons in brain slices from rodents and non-human primates of different ages. In this particular case, we use adult mice (3-6 months old) and adult cynomologus monkeys (9-15 years old). This technique, originally described by the laboratory of Dr. Lichtman (Gan et al., 2000), is well suited for the study of dendritic branching and dendritic spine morphology and can be combined with traditional immunostaining, if detergents are kept at a low concentration.

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