Mammalian Cell Culture
Mammalian Cell Culture - Related Videos
02:45
Production and Collection of Pseudovirus Particles Using Mammalian Cell Culture
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2026
Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the procedure for infecting mammalian cells with green fluorescent protein (GFP)-encoding pseudovirus, monitoring viral replication and spread by fluorescence microscopy, and collecting virus-containing supernatant for downstream applications.
09:30
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
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Cited by 17 •
2015
Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.
02:29
Antibody Production from a Batch Overgrowth Culture of Transfected Mammalian Cells
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2025
The video demonstrates a method for producing therapeutic antibodies using stably transfected mammalian cells. Tryptone supplementation stimulates cell division, resulting in overgrowth and supporting a higher antibody yield. The antibody-containing medium is harvested, filtered, and stored for further use.
11:03
Massively Parallel Reporter Assays in Cultured Mammalian Cells
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Cited by 63 •
2014
The genetic reporter assay is a well-established and powerful tool for dissecting the relationship between DNA sequences and their gene regulatory activities. Coupling candidate regulatory elements to reporter genes that carry identifying sequence tags enables massive parallelization of these assays.
08:39
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Cited by 4 •
2011
Cells play an instrumental and increasing role in research, and the discovery and development of new therapeutics. With this increasing need for greater number of cells we need more efficient and effective ways for growing and harvesting attachment dependent cells. A Multilayered flask with the right features can serve this purpose.
FAQs
The medium supplies the basic inputs required for cell maintenance and growth, including energy, amino acids, salts, and growth factors. These components support continued cell activity and help sustain the productivity needed in engineered biological systems. Because medium composition directly provides these resources, it is a central part of maintaining viable and functional cultures.
Incubators regulate temperature, pH, and gases to keep the culture environment controlled. This environmental control helps preserve cell viability and supports consistent productivity during growth and maintenance. In engineering workflows, regulating these conditions also contributes to reproducibility, making results more dependable across experiments or production processes.
Adherent cells must attach to a vessel surface, while suspension cells remain freely distributed in the culture medium. This difference affects how each population is maintained within the vessel and provides two distinct formats for engineered biological work. The choice of format can therefore align the culture system with the intended application or process design.
A basic workflow places the cells in a sterile vessel containing nutrient medium, then maintains them in an incubator that regulates temperature, pH, and gases. The culture is supported as cells grow and are maintained under these controlled conditions. This combination of sterility, nutrient supply, and environmental regulation underpins consistent operation.
Engineering applications include recombinant protein production, tissue engineering, drug testing, and biomanufacturing. In these settings, the culture provides a controlled biological platform for developing or evaluating engineered systems. Its value extends from producing biological products to supporting tissue-related designs and testing approaches intended for pharmaceutical or manufacturing use.
Process control helps maintain viability, productivity, and reproducibility throughout mammalian cell culture. Viability reflects whether cells remain capable of continued maintenance, productivity concerns the desired output of the culture, and reproducibility supports comparable results between runs. Together, these outcomes make the system more useful for research, development, and biomanufacturing.