Yeast Hypoxic Response
Yeast Hypoxic Response - Related Videos
09:45
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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Cited by 2 •
2017
Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.
08:47
The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia
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Cited by 48 •
2008
The postnatal rat model for hypoxic-ischemic brain injury is a well-established model of human neonatal hypoxic ischemic encephalopathy (HIE). In this article, we describe the model of HIE in post-natal rat pups.
02:52
Neuronal Adaptation to Repetitive Hypoxic Preconditioning in Mice
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2025
Source: Poinsatte, K., et al., Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice. J. Vis. Exp. (2015)This video demonstrates the method of repetitive hypoxic preconditioning (RHP) by exposing a mouse to controlled hypoxia cycles before inducing an ischemic stroke. The experiment evaluates its effects on blood-brain barrier stability, neuronal adaptation, and inflammation.
03:09
Establishing a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
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2025
The video demonstrates the procedure for creating a mouse model to study neonatal hypoxic-ischemic brain injury. It outlines the steps for surgically inducing ischemia and subjecting the animal to hypoxia to exacerbate brain damage. Finally, it shows how to assess the resulting brain lesions, ensuring the mouse model is prepared for further scientific investigation.
08:30
Yeast Maintenance
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2023
Research performed in the yeast Saccharomyces cerevisiae has significantly improved our understanding of important cellular phenomona such as regulation of the cell cycle, aging, and cell death. The many benefits of working with S. cerevisiae include the facts that they are inexpensive to grow in the lab and that many ready-to-use strains are now commercially available. Nevertheless, proper maintenance of this organism is critical for successful experiments. This video will provide an overview...
FAQs
Oxygen limitation changes transcription by removing repression from oxygen-sensitive regulatory networks. As oxygen and sterol availability decline, factors such as Rox1 no longer maintain the same level of repression, allowing hypoxia-responsive genes to be expressed. This transcriptional shift helps redirect cellular activity toward anaerobic lipid and sterol metabolism rather than oxygen-dependent processes.
Rox1 and Upc2 contribute opposing regulatory activities during oxygen limitation. Reduced oxygen and sterol availability relieve repression associated with Rox1, while Upc2 becomes activated and promotes transcription of genes needed for anaerobic lipid and sterol metabolism. Their coordinated behavior illustrates how regulatory proteins connect environmental sensing with specific genetic and metabolic adjustments.
Sterol availability functions with oxygen status as an important signal for transcriptional regulation. When both become limited, repression is relieved and regulators such as Upc2 activate genes supporting anaerobic lipid and sterol metabolism. This relationship shows that the response is not controlled by oxygen alone, but by linked changes in membrane-related resources and cellular physiology.
The response demonstrates that genetic regulation can reorganize metabolism when environmental conditions change. Oxygen-sensitive transcription factors alter which genes are expressed, enabling yeast to adjust lipid and sterol metabolism while also modifying growth-related physiology. In genetics, this provides a tractable example of how cells convert an external limitation into coordinated changes in gene activity and metabolism.
Yeast offers a tractable model for connecting oxygen availability with transcriptional regulation, metabolism, and growth. Researchers can examine how regulatory networks involving Rox1 and Upc2 influence hypoxia-responsive genes without separating genetic effects from broader cellular adaptation. Findings from this system help clarify general principles of gene regulation and environmental response in a manageable biological context.
Fermentation performance depends partly on oxygen availability, which can influence yeast metabolism, growth, and cell performance. Understanding the hypoxic response helps researchers interpret how oxygen limitation changes transcription and anaerobic lipid and sterol metabolism. That knowledge can support efforts to improve fermentation processes by relating oxygen conditions to productivity and the physiological state of the cells.