Dead Cell Marker
Dead Cell Marker - Related Videos
10:47
Quantification of Proliferative and Dead Cells in Enteroids
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Cited by 2 •
2020
The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.
09:48
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
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Cited by 7 •
2021
Neurodegenerative diseases are associated with dysregulated microglia functions. This article outlines an in vitro assay of phagocytosis of neuroblastoma cells by iPSC-macrophages. Quantitative microscopy readouts are described for both live-cell time-lapse imaging and fixed-cell high-content imaging.
10:56
Co-localization of Cell Lineage Markers and the Tomato Signal
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Cited by 18 •
2016
We developed two sets of tracing combinations in Rosa26tdtomato (ubiquitously expressed in all cells)/Cre (specifically expressed in chondrocytes) mice: one with 2.3Col1a1-GFP (specific to osteoblasts) and one with immunofluorescence (specific to bone cells). The data demonstrate the direct transformation of chondrocytes into bone cells.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
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2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
FAQs
The key change is loss of plasma-membrane integrity. Once the barrier is sufficiently damaged, a membrane-impermeant dye can enter the cell and bind intracellular nucleic acids, generating a measurable signal. The readout therefore reflects physical membrane damage rather than simply diminished cellular activity, helping investigators avoid labeling functionally impaired but still viable cells as dead.
A marker identifies nonviability, whereas reduced function alone does not establish that a cell has died. Pairing the signal with a functional readout helps separate actual cell loss from cells whose activity has declined after immune activation, infection, or treatment. That distinction improves interpretation of cytotoxicity and immune-cell responses.
Dead-cell-marker measurements can support classification into live, dead, and sometimes dying populations, depending on the marker and assay context. This is valuable when an experiment examines a transition rather than only an endpoint. Reporting these categories can show whether infection, immune activation, or treatment is associated with completed cell death or an intermediate state.
Flow cytometry is useful for measuring marker-positive and marker-negative cell populations, whereas microscopy links the signal to individual cells and their visual context. Viability assays provide another way to quantify the outcome. The choice depends on whether the study prioritizes population-level measurement, cell-level observation, or a general viability readout.
After infection, dead-cell markers help quantify damage associated with the pathogen rather than relying only on changes in cell activity. Applying the readout to infected samples can reveal the extent of nonviability and provide evidence for pathogen-induced injury. In immunology studies, that information helps evaluate how infection alters the survival of relevant cell populations.
Following immune activation or treatment, the assay can help determine whether an observed loss of performance reflects cytotoxicity or reduced function without cell death. That distinction matters when interpreting immune-cell responses, because a weaker response does not by itself prove that cells are nonviable. The marker therefore adds a direct viability-related measure to the experimental readout.