Dendritic Cell Trafficking
Dendritic Cell Trafficking - Related Videos
11:27
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
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Cited by 16 •
2016
We describe a modified method for membrane fraction isolation from human cells and sample preparation for the detection of SCAP N-glycosylation and total protein by using western blot. We further introduce a GFP-labeling method to monitor SCAP trafficking using confocal microscopy. This protocol can be used in regular biology laboratories.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
09:22
Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
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Cited by 6 •
2010
Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.
FAQs
Tissue-derived microbial products and inflammatory cytokines alter dendritic cell behavior by inducing chemokine receptors and adhesion molecules. These changes help the cells respond to directional signals, interact with the extracellular matrix, and move toward lymphatic vessels. The resulting relocation connects local detection of infection with access to lymph nodes, where adaptive immune activation can begin.
Chemokine receptors help dendritic cells respond to positional signals that guide movement, while adhesion molecules support interactions needed for passage through tissues and across lymphatic endothelium. Their coordinated induction is important because migration requires more than directional sensing. Cells must also move through the extracellular matrix and enter lymphatic vessels before reaching a lymph node.
Entry into lymphatic vessels provides a route from peripheral tissues to lymph nodes. This transition brings dendritic cells carrying processed antigen into the anatomical setting where naïve T cells can encounter antigen-presenting cells. Consequently, lymphatic passage is a functional link between events occurring at an infected tissue and the initiation of a coordinated adaptive immune response.
The process begins with tissue signals generated by microbial products and inflammation, reflecting innate recognition of danger. Those signals promote movement toward lymph nodes, where dendritic cells present processed antigen to naïve T cells. Trafficking therefore coordinates two stages of immunity: detecting conditions in peripheral tissues and communicating antigen-specific information to cells that can launch adaptive responses.
A useful conceptual sequence follows dendritic cells from tissue activation through extracellular-matrix movement, lymphatic endothelial crossing, and arrival in lymph nodes. The final stage includes antigen presentation to naïve T cells. Examining these linked transitions helps researchers determine how inflammatory signals shape migration and how movement influences the start of adaptive immune activation.
Because lymph-node arrival places antigen-presenting dendritic cells near naïve T cells, trafficking provides a relevant framework for understanding how immune responses are initiated. Vaccine research can use this relationship to consider movement toward lymph nodes, while cancer immunotherapy can examine the same antigen-presentation pathway. The process therefore connects tissue localization with strategies intended to strengthen immune activation.
Trafficking helps determine how signals from tissues are carried into lymph nodes and converted into adaptive immune activation. If this coordination is altered or persistently engaged, the pathway becomes relevant to conditions involving chronic inflammation or autoimmunity. Studying the movement process can therefore support strategies aimed at controlling inappropriate or sustained immune responses, in addition to responses to infection.