Facs Tube
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06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
10:56
Tube Thoracostomy
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2023
Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Tube thoracostomy (chest tube placement) is a procedure during which a hollow tube is inserted into the thoracic cavity for drainage of fluid or air. Emergency chest tube insertion is performed for definitive treatment of tension pneumothorax, traumatic hemothorax, large-volume pleural effusions, and empyemas. Irrespective of the cause of air and fluid accumulation in the pleural space, the...
11:04
Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
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2023
Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France Adoptive cell transfer is a method for introducing cells into a patient or study organism...
10:50
Isolation of Pericytes from Mouse Cortical Tissue Using FACS for Single-cell Sequencing
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Cited by 1 •
2026
To obtain mouse brain cortical pericytes meeting the demands of scRNA-seq, we present a critically optimized FACS protocol based on CD13⁺/CD31⁻ selection. Our key refinements significantly enhance cell viability and RNA integrity, enabling reliable transcriptomic profiling.
FAQs
Fluorescently labeled antibodies bind selected markers on or within cells during incubation. After labeling, the cells are washed to remove unbound reagent and resuspended for measurement. This preparation links marker recognition to laser-based fluorescence detection, allowing the instrument to distinguish cells according to the labeled surface or intracellular features being studied.
Washing has a specific analytical purpose: it reduces fluorescent antibody that has not associated with cells. If excess reagent remains in the suspension, it can complicate interpretation of fluorescence measurements. Resuspension after washing places the prepared cells into a suitable suspension for entry into the cytometer, supporting clearer identification of labeled populations.
Hydrodynamic focusing aligns individual cells for laser-based detection. A low-clumping suspension helps preserve this single-cell presentation, whereas aggregates may prevent reliable assignment of measured fluorescence to individual cells. Careful sample handling and preparation in the tube therefore support cleaner measurements and improve the ability to identify or sort defined cell populations.
A practical preparation sequence starts by placing the cell suspension in a correctly labeled FACS tube, incubating it with fluorescently labeled antibodies, washing away unbound reagents, and resuspending the cells. The prepared tube is then transferred to the flow instrument, where cells pass through laser detection after hydrodynamic focusing.
Correct labeling and careful sample handling are central to reliable FACS tube use. Clear identification preserves the connection between each suspension and its intended sample, while minimizing clumping helps the instrument evaluate individual cells. These practices matter especially when multiple immune-cell or infection-related populations are prepared for comparison, sorting, or downstream study.
They are useful when investigators need to identify or sort immune-cell populations, pathogen-infected cells, or cells expressing selected surface or intracellular markers. Following analysis or sorting, the recovered populations can support downstream functional or molecular studies. The tube therefore forms part of a workflow connecting fluorescent phenotyping with further biological investigation.