Mouse Liver Repopulation
Mouse Liver Repopulation - Related Videos
09:13
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
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Cited by 7 •
2016
The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.
11:44
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Cited by 3 •
2016
Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.
03:42
Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
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2025
In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.
09:43
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
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Cited by 17 •
2015
This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
FAQs
A selective growth advantage allows donor hepatocytes to expand more effectively than remaining recipient cells after liver injury or hepatocyte loss. This difference in growth potential drives progressive donor-cell engraftment and replacement rather than simple short-term cell persistence. The resulting system is useful for examining whether introduced cells can contribute substantially to liver mass and function.
Endogenous hepatocyte injury or loss creates the tissue conditions that favor donor-cell expansion. Without this selective pressure, introduced cells may not progressively occupy a substantial portion of the liver. The extent and nature of the recipient-cell deficit therefore influence how effectively donor hepatocytes engraft, proliferate, and participate in restoring hepatic tissue.
The model permits comparison of liver responses when hepatocytes are transplanted, genetically modified, or associated with infection-related changes. Host immune activity can then be examined in relation to tissue damage and the persistence or performance of those cells. This connects hepatocyte behavior with immune-mediated injury in a regenerating liver environment.
Progressive replacement by donor hepatocytes provides evidence that introduced cells can expand within injured liver tissue and contribute to restoration of liver mass and function. The system therefore links cellular engraftment with regenerative capacity, rather than evaluating donor cells only as an isolated transplant. It supports investigation of how liver tissue recovers after hepatocyte loss.
A conceptual workflow includes establishing recipient-liver injury or hepatocyte loss, introducing donor hepatocytes through transplantation or genetic introduction, and allowing selective expansion within the liver. Researchers can then evaluate the extent of donor-cell replacement, liver mass restoration, and functional contribution. The sequence makes the model suitable for studying both engraftment and tissue regeneration.
Researchers can use the system to assess host responses toward infected or genetically modified hepatocytes and to examine immune-mediated tissue damage. Because donor cells expand within the liver, the model connects immune activity with hepatocyte persistence, replacement, and hepatic function. It therefore provides a context for studying interactions between altered liver cells and the recipient immune system.
Repopulation creates an experimental setting for asking whether introduced hepatocytes can engraft, expand, and restore liver mass or function. These outcomes help assess the performance of cell-based therapies beyond initial delivery. The approach is especially relevant when therapeutic benefit depends on donor cells becoming a substantial, functioning component of the recipient liver.
The system supports therapeutic strategies for inherited liver disorders by providing a way to study genetically introduced or transplanted hepatocytes within recipient liver tissue. Researchers can examine whether these cells expand sufficiently to replace damaged or deficient hepatocytes and contribute to hepatic restoration. This makes repopulation relevant to evaluating regenerative and cell-based approaches for inherited disease.