Surgical Isolation

Surgical isolation is an infection-control approach that separates a surgical patient, operative field, or procedure from potential sources of microbial contamination, protecting both the patient and healthcare personnel. It works by combining physical barriers, dedicated equipment, controlled movement, and aseptic technique to limit the transfer of infectious agents before, during, and after an operation. In immunology and infection research, these measures help reduce surgical-site contamination, prevent transmission of pathogens, and preserve the integrity of clinical specimens and experimental observations. Proper isolation planning also supports safer care when patients carry transmissible infections or when procedures involve materials requiring strict containment.

Surgical Isolation - Related Videos

Research

JoVE Journal - Medicine

Surgical Perfusion and Isolation of the Porcine Pancreas for Islet Isolation

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Cited by 1 •

2024

This protocol provides a step-by-step guide for the procurement of a porcine pancreas for islet isolation and purification.

Neonatal Mouse Ovary Isolation: A Surgical Procedure to Harvest Pair of Ovaries from Neonatal Mouse Model

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2025

In this video, we describe a surgical procedure to isolate ovaries from a neonatal mouse.

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

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Cited by 44 •

2014

Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.

Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model

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2025

In this video, we describe a surgical procedure to harvest intact ovary from a mouse model.

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

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Cited by 11 •

2015

A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.

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