Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

M. Yakut Ali; Sandeep V. Anand; Krishnarao Tangella; Davendra Ramkumar; Taher A. Saif

doi:10.3791/52532

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Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

15,503 Views

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09:28 min

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June 04, 2015

DOI:

10.3791/52532-v

DOI:

10.3791/52532-v

•

09:28 min

June 04, 2015

•

7 Views

M. Yakut Ali¹, Sandeep V. Anand¹, Krishnarao Tangella^2,3, Davendra Ramkumar^2,3, Taher A. Saif^1,4

¹Department of Mechanical Science and Engineering,University of Illinois at Urbana-Champaign, ²College of Medicine,University of Illinois at Urbana-Champaign, ³Provena Covenant Medical Centre, ⁴Micro and Nanotechnology Laboratory,University of Illinois at Urbana-Champaign

Summary

A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.