Cholestasis Research Model
Cholestasis Research Model - Related Videos
04:38
Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis
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Cited by 22 •
2018
Here, we present partial bile duct ligation as a surgical model of liver injury and regeneration in rodents.
09:39
Creation of Reversible Cholestatic Rat Model
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2011
Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.
08:56
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
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Cited by 300 •
2015
Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28 days.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
07:57
Scaled Anatomical Model Creation of Biomedical Tomographic Imaging Data and Associated Labels for Subsequent Sub-surface Laser Engraving (SSLE) of Glass Crystals
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2017
A methodology is described herein for representing anatomical imaging data within crystals. We create scaled three-dimensional models of biomedical imaging data for use in Sub-Surface Laser Engraving (SSLE) of crystal glass. This tool offers a useful complement to computational display or three-dimensionally printed models used within clinical or educational settings.
FAQs
Models can be organized around disruptions in hepatocyte transporters, bile duct function, or regulatory pathways controlling bile acid synthesis, secretion, and clearance. Comparing these mechanisms helps determine how impaired handling produces accumulation of biliary substances and contributes to downstream liver injury. This framework connects a specific experimental disturbance with the biological processes involved in cholestatic disease.
Cellular, organoid, and animal systems provide complementary ways to investigate cholestatic injury. They can be used to examine altered bile handling, hepatotoxicity, inflammation, fibrosis, and liver repair across experimental settings. Including these model categories broadens the research question from a molecular or cellular mechanism to more complex patterns of tissue injury and recovery relevant to medicine.
These pathways represent distinct points at which bile acid balance can be disturbed. Altered synthesis may change the amount produced, while impaired secretion or clearance can promote retention and accumulation. Examining the pathways separately helps researchers associate a model's initiating defect with its effects on hepatotoxicity and other cholestatic injury processes.
Selection should follow the biological question being tested. A study focused on hepatocyte transporters or bile acid regulation may use a cellular or organoid system, whereas broader investigation of inflammation, fibrosis, or liver repair may require a model that captures more complex liver responses. Matching model scope to the intended outcome improves interpretability.
These models can support biomarker discovery by linking experimental cholestatic injury with measurable biological changes. They also allow investigation of hepatotoxicity, inflammation, fibrosis, and liver repair, creating several outcome categories rather than a single injury measurement. Such findings can help identify signals relevant to cholestatic disease and drug-induced liver injury research.
A cholestasis research model can be used to examine whether a drug-related disturbance affects biliary transport, bile duct function, or bile acid regulation. Researchers can then evaluate associated hepatotoxicity and related responses such as inflammation or fibrosis. This application supports assessment of drug-induced liver injury and can inform the development of therapies for cholestatic liver diseases.