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Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
JoVE Journal
Medicine
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JoVE Journal Medicine
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

DOI:
10.3791/52438-v

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08:56 min

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February 10, 2015

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, , , , , ,
1Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry,RWTH Aachen University, 2Institute for Laboratory Animal Science and Experimental Surgery,RWTH Aachen University, 3Department of Medicine III,RWTH Aachen University

Chapters

  • 00:05Title
  • 01:54Pre-surgical Preparation
  • 03:17Surgical Procedure
  • 05:43Results: Obstructive Cholestasis-induced Inflammatory Liver Injury
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28 days.

Tags

Keywords: Bile Duct LigationMiceCholestasisLiver InjuryLiver FibrosisObstructive CholestasisBile Flow ObstructionSurgical TechniqueAnimal Model

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