Isolated Muscle
Isolated Muscle - Related Videos
14:02
Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
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Cited by 53 •
2012
We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.
09:45
Mitochondrial Isolation from Skeletal Muscle
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Cited by 48 •
2011
This protocol describes a procedure to study the respiration of mitochondria isolated from skeletal muscles. This method was adapted from Scorrano et al. (2007). The mitochondrial isolation procedure requires about 2 hours. The mitochondrial respiration can be completed in about 1 hour.
08:42
Myo-mechanical Analysis of Isolated Skeletal Muscle
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Cited by 25 •
2011
To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.
14:36
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
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Cited by 6 •
2010
The micro-dissected explants technique is a robust and reliable method for isolating proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. Uniquely, these cells have been clonally derived to produce skeletal muscle stem cell lines used for in vivo transplantation.
08:24
Isolation of Murine Coronary Vascular Smooth Muscle Cells
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Cited by 11 •
2016
The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.
FAQs
Stimulation initiates a measurable muscle response while the preparation remains outside the organism. By recording contraction force, shortening, and relaxation, investigators can examine how excitation produces mechanical activity and how that activity resolves afterward. This direct readout helps distinguish changes in contractile performance from broader whole-body influences, supporting studies of impaired motor function and muscle disease.
These conditions help preserve a controlled experimental environment and establish a consistent baseline for measurement. Oxygenated physiological solution supports the tissue while it is maintained in the organ bath, and appropriate temperature and resting tension help standardize its mechanical state. If these parameters are not maintained appropriately, comparisons of contraction or relaxation responses may become less reliable.
The two approaches provide complementary information. Electrical stimulation can be used to evoke a muscle response directly, whereas nerve stimulation includes the pathway that communicates with the muscle. Comparing their recorded contractions can therefore help investigators examine muscle performance alongside neuromuscular transmission, an important distinction when studying altered motor function or drug effects.
Researchers remove the muscle tissue, place it in an organ bath containing oxygenated physiological solution, and mount it under appropriate resting tension and temperature. They then apply electrical or nerve stimulation and record the resulting contraction force, shortening, or relaxation. This sequence creates controlled conditions for evaluating muscle behavior and responses to experimental interventions.
The preparation can provide several mechanical indicators, including contraction force, shortening, and relaxation. These measurements allow researchers to assess how strongly the tissue responds, how its length changes during activity, and how it returns toward rest. Repeated observations can also support investigation of fatigue and reveal changes associated with drugs or disease-related muscle dysfunction.
This preparation is valuable when researchers need to examine muscle or neuromuscular function without the complexity of the whole body. Medical and pharmacological studies can use it to investigate muscle disorders, anesthetic and therapeutic agents, fatigue, and impaired motor function. Its controlled setting helps link an intervention or disease-related change to a measurable tissue response.