Colorectal Tissue Isolation
Colorectal Tissue Isolation - Related Videos
06:19
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
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Cited by 13 •
2017
We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.
03:53
Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model
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2023
The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.
03:50
CTC Isolation from a Whole Blood Sample: A Method to Obtain CTCs from Murine Colorectal Cancer Model
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2023
This video describes a detailed protocol for isolating circulating tumor cells from the whole blood sample of a euthanized colorectal tumor mouse model. These isolated CTCs can be cultured and used for intended downstream analysis.
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
05:19
Segmental Adeno-Cre Infection: A Technique to Generate Isolated Colorectal Cancer using Genetically Engineered Mouse Models
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2023
This video describes the generation of a colorectal mouse model by segmental adeno-cre infection. The infected colon segment leads to tumorigenesis within this segment of the mucosa, resulting in adenomas ultimately progressing to invasive and metastatic carcinoma. This model is an attractive platform for studies of cancer biology and preclinical therapeutic trials.
FAQs
The main preparation goal is to match tissue handling with the intended analysis. If histological work requires preserved architecture, dissection and processing must maintain the tissue's organized structure. For cellular studies, the emphasis may shift toward releasing defined populations while retaining cell viability. This distinction helps researchers interpret whether a result reflects tissue organization or isolated cell behavior.
Washing and trimming are preparatory controls that refine material before downstream work. Together with careful dissection, they help establish a consistent starting sample without committing the study to cell dissociation. This flexibility matters because some analyses require preserved architecture, whereas others need released cell populations for analysis.
Mechanical and enzymatic dissociation are used when the study needs cells released from the prepared tissue. They are not required for every endpoint, because some investigations depend on preserving tissue architecture. Selecting whether to dissociate therefore aligns sample processing with cellular analysis, histology, or other downstream goals.
Sterile handling and controlled conditions help limit contamination and degradation, which directly affects the trustworthiness of downstream results. Their importance extends beyond cell isolation: damaged or contaminated material may weaken cellular, molecular, or histological analysis. Maintaining these controls therefore supports more reliable comparisons across colorectal samples.
A typical workflow begins with careful dissection, followed by washing and trimming. If the downstream study requires separated cell populations, the prepared material may then undergo mechanical or enzymatic dissociation. The sequence is adjusted to protect either tissue architecture or cell viability, producing material suited to the planned analysis.
Isolated colorectal tissue can support studies of epithelial biology, inflammation, cancer, barrier function, and patient-derived models. Depending on preparation, investigators may use it for organoids or ex vivo assays, linking tissue handling to questions about disease biology and therapeutic response in medicine and translational research.
Standardized isolation improves sample quality and reproducibility by making preparation more consistent across experiments. That consistency strengthens translational studies of gastrointestinal disease because findings are less dependent on uncontrolled differences in tissue processing. It also supports evaluation of therapeutic response when samples are prepared comparably.