Immunofluorescent Antibody Staining
Immunofluorescent Antibody Staining - Related Videos
09:56
Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections
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2023
Source: Thomas Chaffee1, Thomas S. Griffith2,3,4, and Kathryn L. Schwertfeger1,3,4 1 Department of Lab Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455 2 Department of Urology, University of Minnesota, Minneapolis, MN 55455 3 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 4 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 Pathologic analyses of tissue sections can be used to obtain a better understanding of normal tissue...
08:35
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
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Cited by 2 •
2017
Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.
10:13
Method for Whole Mount Antibody Staining in Chick
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Cited by 3 •
2009
This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.
13:10
Antibody Staining in C. Elegans Using "Freeze-Cracking"
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Cited by 34 •
2013
"Freeze-cracking," a method for exposing the inner tissues of the nematode C. elegans to antibodies for protein localization, is demonstrated.
03:24
BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
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2023
This video describes the protocol for BrdU immunofluorescence staining. The technique allows the identification of cells in different phases of cell cycle. Addition of DNA dyes and antibody labeling allows the detailed analysis of the fate of the S phase cells at later times.
FAQs
Direct and indirect staining differ in how fluorescence is attached to the antibody system. Direct staining places the fluorophore on the primary antibody, whereas indirect staining uses an unlabeled primary antibody and a fluorescent secondary antibody. This choice determines which antibody participates in target recognition and how the signal is generated for microscopy.
In indirect staining, the secondary antibody recognizes the unlabeled primary antibody after that primary antibody has bound the antigen. Because the secondary antibody carries the fluorophore, it provides the link between antigen recognition and fluorescence detected by the microscope. Its role therefore depends on the primary-secondary antibody pairing.
Locating a fluorescent signal within cells, tissues, or clinical specimens helps relate a molecular target to the specimen’s structural context. This relationship is important because the technique can do more than indicate that an antigen is present: it can support examination of how proteins, infectious agents, or immune-related targets are positioned within studied material.
A practical staining workflow must coordinate the clinical specimen or tissue sample with the antibody system and the imaging step. The selected primary antibody supplies target recognition; indirect assays additionally require a compatible fluorescent secondary antibody. A fluorescence microscope then allows the labeled target to be examined in its cellular or tissue setting.
It can reveal where an infectious agent or its associated antigen is located in a clinical specimen. That spatial information adds context beyond identifying a molecular target alone, because the observed signal can be considered alongside the organization of cells or tissue. The method therefore supports investigation of infection within a structured biological sample.
Immunofluorescent Antibody Staining supports evaluation by showing the location of relevant targets in cells, tissues, or clinical specimens. Researchers and clinicians can use that visual evidence to examine immune-associated findings in relation to tissue structure, helping connect molecular observations with disease processes rather than considering the target separately from its biological setting.