Intestinal Pathology
Intestinal Pathology - Related Videos
09:01
Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
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Cited by 9 •
2015
We describe methods for longitudinal monitoring of the efficacy of therapeutics for the treatment of colonic pathologies in mice using a rigid endoscope. This protocol can be readily used for the characterization of the therapeutic response of an individual tumor in live mice and also for monitoring potential disease relapse.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
09:25
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
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Cited by 68 •
2012
Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.
FAQs
Damage to the intestinal epithelium can alter barrier function, allowing local injury and immune activation to become linked processes. Pathological assessment therefore considers how epithelial abnormalities relate to inflammation and tissue repair rather than viewing each change in isolation. This relationship helps connect microscopic findings with symptoms, disease progression, and the mechanisms producing intestinal damage.
Immune activation and infection can produce overlapping abnormalities in intestinal tissue, so their contribution must be considered alongside epithelial injury and altered barrier function. Comparing these mechanisms with the clinical and endoscopic picture helps pathologists interpret inflammatory patterns more accurately. This is particularly relevant when distinguishing infectious colitis from inflammatory bowel disease, which may require different clinical decisions.
Vascular changes can influence the pattern and severity of intestinal injury, while abnormal tissue repair may shape the subsequent structural appearance of affected tissue. Together, these processes help explain why intestinal lesions can evolve over time rather than remaining static. Recognizing them supports interpretation of ischemic injury and improves links between microscopic patterns and disease progression.
Interpretation begins by relating findings in intestinal biopsies or surgical specimens to the patient’s clinical presentation and endoscopic observations. Histopathology supplies microscopic evidence, while clinical and endoscopic information provides the broader disease context. Combining these sources improves diagnostic accuracy because the same general tissue abnormality may have different significance in inflammatory, infectious, vascular, immune-mediated, or neoplastic conditions.
Pathological assessment can help separate inflammatory bowel disease, infectious colitis, ischemic injury, celiac disease, and intestinal neoplasms. The distinction depends on recognizing characteristic microscopic patterns and interpreting them with clinical and endoscopic findings. This comparative approach is important because identifying the disease category can clarify the likely process involved and support appropriate treatment decisions.
In clinical medicine, intestinal pathology helps connect tissue abnormalities with symptoms, progression, diagnosis, and treatment planning. Its value extends across inflammatory, infectious, ischemic, immune-mediated, and neoplastic disease contexts. In research, examining mechanisms of intestinal injury, including barrier disruption, immune activation, vascular change, and tissue repair, can support investigations into how disease develops and evolves.