Intravital Two Photon Microscopy
Intravital Two Photon Microscopy - Related Videos
10:08
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
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Cited by 13 •
2012
We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.
09:00
Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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Cited by 21 •
2014
The extracellular matrix undergoes substantial remodeling during wound healing, inflammation and tumorigenesis. We present a novel intravital immunofluorescence microscopy approach to visualize the dynamics of fibrillar as well as mesh-like matrix components with high spatial and temporal resolution using epifluorescence or two-photon microscopy.
10:07
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
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2014
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
07:25
Two-Photon Intravital Microscopy of Glioblastoma in a Murine Model
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Cited by 2 •
2024
We present a novel approach for two-photon microscopy of the tumor delivery of fluorescent-labeled iron oxide nanoparticles to glioblastoma in a mouse model.
09:52
An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
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Cited by 24 •
2014
We have established a cortical orthotopic glioblastoma model in mice for intravital two-photon microscopy that recapitulates the biophysical constraints normally at play during the growth of the tumor. A chronic glass window replacing the skull above the tumor enables the follow-up of the tumor progression over time by two-photon microscopy.
FAQs
The pulsed near-infrared beam produces two-photon fluorescence excitation only at the microscope’s focal point. Because excitation is spatially restricted, regions above and below that point receive less excitation and experience less out-of-focus photodamage. This selectivity helps investigators observe cellular and molecular events within living tissue while preserving the surrounding biological environment.
Near-infrared illumination enables imaging deeper below tissue surfaces than surface-focused observations alone. Combined with focal excitation, it supports visualization of events occurring within intact living tissues rather than only in exposed or isolated samples. This makes the approach useful when disease-related behavior depends on native tissue organization and physiological surroundings.
Limiting out-of-focus photodamage helps distinguish biological changes from damage caused by the imaging process itself. The resulting observations are better suited to examining disease progression, cellular behavior, and treatment effects in living tissue. This is especially important when investigators need to follow dynamic processes over time instead of collecting only a single endpoint.
The experiment requires a living organism or living tissue context, a microscope capable of focal two-photon fluorescence excitation, and a pulsed near-infrared laser source. Imaging is performed at selected focal points below the tissue surface, allowing investigators to examine events in their physiological setting. These conditions connect microscopic observations with ongoing biological processes.
In medicine, the technique can follow immune-cell migration, tumor growth, blood-vessel behavior, and responses to therapy. Tracking these processes over time reveals how cells and tissues change during disease progression or treatment. Rather than providing only a static image, repeated observation can link microscopic dynamics with evolving clinical or experimental outcomes.
By visualizing tissue behavior during treatment, intravital two-photon microscopy can show how therapy affects tumors, immune-cell movement, or blood vessels. These microscopic observations help connect treatment exposure with biological response in the native tissue environment. The method therefore supports interpretation of whether disease-associated processes change as therapy takes effect.