Laser Protocol
Laser Protocol - Related Videos
09:43
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
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Cited by 27 •
2013
Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.
14:18
Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
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Cited by 2 •
2016
A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions.
07:35
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
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Cited by 4 •
2021
A robust protocol is presented here for isolating neuromelanin granules from human post-mortem substantia nigra pars compacta tissue via laser microdissection. This revised and optimized protocol massively minimizes the required time for sample collection, reduces the required sample amount, and enhances the identification and quantification of proteins by LC-MS/MS analysis.
09:59
An “All-laser” Endothelial Transplant
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Cited by 5 •
2015
An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.
10:44
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
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Cited by 12 •
2018
The goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including relatively simple strand breaks and complex damage, to study DNA damage signaling and repair factor assembly at damage sites in vivo.
FAQs
Wavelength determines which tissue target absorbs the laser energy most strongly. Water, pigment, and hemoglobin can therefore respond differently to the same general treatment approach. Selecting a wavelength according to the intended target helps direct energy toward cutting, coagulation, ablation, or photobiomodulation, while reducing exposure of tissue that should remain untreated.
Power, pulse duration, and spot size control how laser energy is distributed and how much heating occurs in tissue. Their combined settings can favor controlled heating, vaporization, or more selective treatment. Because changing one parameter can alter the overall tissue response, protocols specify these variables together rather than treating them as independent settings.
Selective treatment concentrates laser effects on the intended tissue target while limiting unwanted injury nearby. Absorption by water, pigment, or hemoglobin provides the physical basis for this targeting, but the result also depends on energy settings and tissue characteristics. This principle supports precise clinical effects and helps explain why a protocol must be adapted rather than copied unchanged.
The clinician should confirm the treatment goal, device, wavelength, power, pulse duration, spot size, delivery method, and safety measures. The protocol should then be adjusted for the indication, tissue characteristics, and clinical setting. Reviewing these elements before treatment helps align the intended tissue effect with the available equipment and reduces the risk of unwanted injury.
Safety measures should address both the patient and the staff exposed to the procedure. A complete plan specifies how the laser will be delivered and includes precautions appropriate to the device and clinical setting. These measures are not separate from treatment design: they help clinicians pursue cutting, coagulation, ablation, or photobiomodulation while limiting unintended exposure and tissue damage.
Depending on its settings and treatment goal, a protocol can support tissue cutting, coagulation, ablation, or photobiomodulation. The relevant approach changes with the tissue target and desired biological effect. This range makes protocol selection important in medicine, because the same device category may be configured for different procedures rather than used with one universal set of settings.
Standardized plans record the treatment goal, device settings, delivery method, and safety measures so clinicians can reproduce an intended approach. Reproducibility helps connect specific energy conditions with outcomes such as cutting, coagulation, ablation, or photobiomodulation. Consistency does not eliminate clinical judgment, since the protocol still requires adaptation to tissue characteristics, indication, and setting.