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Genetics

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Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
 

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Article DOI: 10.3791/56213 10:44 min January 31st, 2018
January 31st, 2018

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Summary

The goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including relatively simple strand breaks and complex damage, to study DNA damage signaling and repair factor assembly at damage sites in vivo.

Transcript

Tags

Laser Microirradiation DNA Damage DNA Repair DNA Damage Signaling Repair Factor Assembly Strand Breaks Complex Damage Macromolecular Dynamics Cell Nucleus Cellular Responses Factor Recruitment Modifications Fluorescently-tagged Proteins Hela Cells Culture Medium
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