Mouse Platform Tilting
Mouse Platform Tilting - Related Videos
02:32
Generation of Tilted Amygdala Slices from a Mouse Brain
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2025
The video demonstrates the generation of tilted amygdala slices from a mouse brain. The brain is positioned at an angle on the vibratome specimen stage to obtain slices containing the amygdala and axons from the medial prefrontal cortex that form synaptic connections with the amygdala. The brain slices are maintained at the physiological temperature to preserve the tissue for further experiments.
14:09
Tilt Testing with Combined Lower Body Negative Pressure: a "Gold Standard" for Measuring Orthostatic Tolerance
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Cited by 31 •
2013
We describe a "gold standard" for evaluating orthostatic tolerance (OT) using tilt testing with combined lower body negative pressure (LBNP). This can be combined with non-invasive evaluations of cardiovascular reflex control. Normal and abnormal responses are defined.
12:10
Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
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Cited by 35 •
2011
This article describes a standard method to get a three-dimensional (3D) reconstruction of biological macromolecules using negative staining electron microscopy (EM). In this protocol, we explain how to get the 3D structure of the Saccharomyces cerevisiae exosome complex at medium resolution using the random conical tilt reconstruction method (RCT).
02:13
Inducing a Spinal Cord Contusion in a Mouse Model Using a Spinal Cord Injury Coaxial Platform
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2025
Source: Elzat, E. Y. Y., et al., Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique. J. Vis. Exp. (2022).This video demonstrates the method for inducing a contusion injury at the T9 level spinal cord in a mouse model, using a spinal cord injury coaxial platform. A weight is dropped on the T9 level spinal cord that creates a contusion that immediately causes tissue discoloration and swelling.
16:46
A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
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Cited by 6 •
2014
We present in this article a novel stretching platform that can be used to investigate single cell responses to complex anisotropic biaxial mechanical deformation and quantify the mechanical properties of biological tissues.
FAQs
Changing the platform’s orientation alters the mouse’s body-position conditions and stimulates sensory systems that detect head and body position. The resulting postural adjustments reflect how the animal integrates those signals to maintain balance and coordinate movement. This makes the response useful for examining vestibular contributions alongside broader motor-control changes in preclinical medical research.
Defined tilt angles and speeds determine the nature of the physical challenge presented during testing. Using controlled values allows researchers to examine responses under consistent conditions and compare changes in postural adjustment, movement, latency, or balance across animals or experimental groups. These parameters therefore help relate behavioral performance to the severity or timing of the imposed orientation change.
Each recorded outcome describes a different aspect of the mouse’s response to altered orientation. Latency captures the timing of a response, movement reflects the accompanying motor adjustment, and loss of balance indicates difficulty maintaining posture. Considering these measures together can provide a broader picture of balance, coordination, and vestibular-related impairment than any single observation alone.
Consistent angles, speeds, and recording procedures reduce variation caused by the testing setup rather than by the animals’ physiology. Standardization supports meaningful comparisons among control and disease-model groups, or between untreated and drug-exposed animals. It also helps researchers track whether behavioral responses change with disease progression or after an experimental intervention.
A typical workflow places the mouse on a motorized platform, applies a predefined tilt at a selected angle or speed, and records the animal’s response. Researchers then examine postural adjustments, movement, response latency, or balance loss. Repeating the same controlled conditions across groups enables behavioral measurements to be compared within a structured experimental design.
The essential setup includes a motorized platform capable of producing controlled orientation changes and a way to record the mouse’s behavioral response. Researchers specify the tilt angle or speed before testing and observe outcomes such as movement, latency, postural adjustment, or balance loss. These defined conditions make the procedure suitable for standardized preclinical measurements.
This approach is useful when investigators need behavioral evidence related to balance, motor coordination, or vestibular function in mice. Applications described for the method include studying inner-ear disorders, neurological disease, motor impairment, and drug effects. The resulting measurements can help connect physiological mechanisms with disease progression and evaluate changes associated with treatment responses.
Changes in postural adjustment, movement, response latency, or balance can provide measurable behavioral indicators across experimental time points or treatment conditions. In disease models, these outcomes may help relate functional impairment to progression. In drug studies, comparing standardized responses can show whether an intervention is associated with altered motor, balance, or vestibular-related performance.