Phosphatidylserine Expression
Phosphatidylserine Expression - Related Videos
04:21
Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling
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2025
In this video, we demonstrate the Annexin V binding assay to measure the degree of eryptosis, erythrocyte programmed cell death, using fluorescent Annexin V staining. Apoptotic erythrocytes demonstrate phosphatidylserine translocation from the inner to the outer leaflet of the cell membrane, causing the fluorophore-tagged Annexin V to bind to the translocated phosphatidylserine, thereby emitting a fluorescent signal.
08:49
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Cited by 1 •
2021
Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro.
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
05:49
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Cited by 2 •
2024
Procoagulant platelet formation has been correlated with an increased risk of thrombosis. Presented here is a precise protocol for isolating washed platelets from human blood, intended to quantify the exposure of phosphatidylserine and microvesicle release, which are distinctive features of procoagulant platelets.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
FAQs
A rise in intracellular calcium changes membrane lipid organization by activating scramblases and inhibiting flippases. Scramblases promote redistribution between membrane leaflets, while reduced flippase activity limits the return of phosphatidylserine to the inner leaflet. This coordinated response increases surface exposure during apoptosis or cellular activation, linking an intracellular signal to an externally detectable membrane state.
The same surface signal can arise in two different medical contexts: apoptosis, where cells undergo programmed death, and cellular activation, including platelet activation. Because both contexts can increase external phosphatidylserine, researchers must relate the membrane finding to the cell type and experimental or clinical setting. This distinction helps separate tissue injury signals from coagulation-related signals.
Surface-exposed phosphatidylserine connects membrane state with coagulation. Its appearance on activated platelets provides a signal associated with platelet participation in blood-clotting processes. Consequently, studying or targeting this exposure can help investigate how cellular activation relates to thrombosis, while distinguishing coagulation-focused questions from those centered on apoptotic or immune-related signaling.
Interpretation depends on whether the detected surface signal is being studied as evidence of apoptosis or platelet activation. In medical research, phosphatidylserine expression therefore functions as a context-sensitive marker rather than a complete diagnosis by itself. Relating the membrane finding to the relevant cell population and disease question supports clearer analysis of tissue injury, thrombosis, or immune regulation.
Surface phosphatidylserine supports research across several medical areas, including tissue injury, thrombosis, cancer, and immune regulation. Its value comes from connecting abnormal membrane signaling with changes in cell status or activation. Investigators can therefore examine the same membrane feature in different disease contexts, while adapting the biological interpretation to the process under study.
Abnormal surface exposure provides a membrane-level feature that diagnostic and therapeutic strategies can target or monitor. In medicine, this approach may help identify cells associated with apoptosis or activated platelets and examine their relationship to disease processes. The broader goal is to use altered membrane signaling as information for studying tissue injury, thrombosis, cancer, or immune regulation.