Stromal Cell Migration
Stromal Cell Migration - Related Videos
08:40
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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Cited by 44 •
2015
The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
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2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
08:52
An Introduction to Cell Motility and Migration
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2023
Cell motility and migration play important roles in both normal biology and in disease. On one hand, migration allows cells to generate complex tissues and organs during development, but on the other hand, the same mechanisms are used by tumor cells to move and spread in a process known as cancer metastasis. One of the primary cellular machineries that make cell movement possible is an intracellular network of myosin and actin molecules, together known as “actomyosin”, which creates a...
03:58
Generating Schwann Cell-Like Cells from Bone Marrow Stromal Cells
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2025
This video demonstrates a method for obtaining Schwann cell-like cells from multipotent bone marrow stromal cells (BMSCs). Initially, the BMSCs undergo hypoxia treatment, followed by directed differentiation into neuronal progenitor cells. Subsequently, specific media and culture conditions are employed to induce the transformation of these progenitor cells into Schwann cell-like cells.
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
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Cited by 28 •
2015
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
FAQs
Direction is established through the combined influence of chemical gradients, physical properties of surrounding tissue, and signals from neighboring cells. These inputs help stromal cells determine where movement should occur rather than simply producing random displacement. Studying how these cues shape movement is important for understanding tissue organization, repair, inflammation, and disease progression.
Stromal cells must modify their immediate surroundings as they move. Remodeling the extracellular matrix creates a changing path through the tissue, while temporary adhesions provide short-lived attachment points between the cell and its environment. Together, these structures help connect cytoskeletal activity to traction, allowing the cell to generate forward movement through surrounding tissue.
Reorganization of the actin cytoskeleton changes the cell’s shape and supports the physical forces required for migration. Actin-based structures work with temporary adhesions to generate traction against the surrounding matrix. This coordination converts directional signals from the environment into cellular movement, linking external chemical or mechanical information with the cell’s ability to advance.
The same migratory behavior that supports tissue repair can contribute to disease when it becomes improperly regulated. Stromal cells influence the organization of healing tissue, inflammatory environments, fibrotic responses, and tumor-associated microenvironments. Consequently, migration studies can help explain how supportive connective-tissue cells participate in both beneficial regeneration and pathological tissue remodeling.
Experimental models provide controlled settings for examining how stromal cells respond to chemical signals, mechanical cues, neighboring cells, and the extracellular matrix. Imaging approaches then reveal cell movement and associated changes in tissue organization. Together, these tools help investigators connect migration behavior with tissue regeneration, repair, fibrosis, inflammation, or tumor-associated stromal activity.
Research commonly connects this process with wound healing, fibrosis, inflammation, and tumor-associated stromal behavior. In wound healing and regeneration, migration relates to tissue organization and repair. In fibrosis and cancer, the same cellular activity helps shape abnormal or disease-associated microenvironments, making the process relevant across several areas of medical investigation.
Migration studies can show how stromal cells influence tissue regeneration and how their movement contributes to abnormal repair or cancer progression. By relating cellular behavior to extracellular-matrix remodeling, cytoskeletal reorganization, and environmental signals, researchers can identify processes that may serve as targets for therapies intended to control harmful tissue responses.