Transplantation Viability Testing
Transplantation Viability Testing - Related Videos
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
12:03
Viability Assays for Cells in Culture
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Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
11:29
A Modified Method for Heterotopic Mouse Heart Transplantion
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Cited by 5 •
2014
A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
FAQs
The assessment draws on several complementary indicators: cellular integrity, metabolic activity, tissue structure, and blood-flow or perfusion responses. Cellular and metabolic findings indicate whether tissue remains active and intact, while structural observations show whether its organization is preserved. Perfusion responses add functional information, helping clinicians interpret whether the organ behaves adequately under preservation conditions.
Ex vivo perfusion evaluates an organ outside the body by circulating oxygenated fluid through it while monitoring physiological performance. This creates an opportunity to observe functional responses during preservation rather than relying only on measurements made during procurement. The resulting information can support decisions about organs that might otherwise be declined and can help tailor transplant planning.
Each measurement examines a different aspect of graft condition. Tissue structure reflects physical preservation, cellular integrity indicates whether cells remain intact, and metabolic activity provides evidence of ongoing function. Blood-flow or perfusion responses add a physiological dimension. Considering these findings together gives clinicians a broader basis for judging suitability than any single type of observation alone.
Viability indicators can be assessed during organ or tissue procurement and throughout preservation. In systems using ex vivo perfusion, monitoring continues while the organ receives oxygenated fluid outside the body. This timing allows clinicians to examine condition before transplantation and to incorporate functional observations into decisions about preservation, acceptance, and individualized transplant planning.
Results can help clinicians distinguish organs with adequate functional potential from those showing concerning preservation or physiological findings. This information supports safer acceptance decisions and may allow selected organs that would otherwise be declined to be considered for transplantation. The approach therefore connects objective laboratory and perfusion observations with allocation choices and anticipated graft performance.
By identifying cellular, metabolic, structural, and perfusion characteristics before transplantation, the assessment can reveal signs associated with poor graft function and guide more informed planning. It also supports evaluation of preservation strategies and may reduce complications linked to poorly functioning grafts. In medicine, this creates a practical link between organ assessment, preservation management, and transplant outcomes.