In Vivo Brain Imaging
In Vivo Brain Imaging - Related Videos
05:32
A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging
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Cited by 11 •
2017
A compact pulsed-laser diode-based photoacoustic tomography (PLD-PAT) system for high-speed in vivo brain imaging in small animals is demonstrated.
02:58
Two-Photon Microscopy for In Vivo Imaging of Mouse Brain Microvasculature
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2025
Source: Nishimura, C. et al. Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation. J. Vis. Exp. (2016). This video demonstrates the in vivo visualization of cortical capillaries in the mouse brain using two-photon microscopy. It outlines the steps for injecting a fluorescent dye into an anesthetized mouse with a thin-skull cortical window, preparing the setup for imaging, and acquiring detailed images of brain...
03:27
In Vivo Imaging of a Rat Brain Using Photoacoustic Tomography
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2025
Source: Upputuri, P. K., et al. A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging. J. Vis. Exp. (2017)This video demonstrates a protocol for in vivo non-invasive rat brain imaging using photoacoustic tomography, showcasing the preparation, imaging system setup, and data acquisition to reconstruct 2D brain images for visualizing vascular structures and neurovascular health.
01:09
Quantitative Susceptibility Mapping of a Human Brain Using Ex-Vivo Magnetic Resonance Imaging
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2025
Quantitative susceptibility mapping, or QSM, quantifies the magnetic susceptibility of brain tissue, which reflects its cellular composition, like myelin content.Begin by acquiring ex-vivo magnetic resonance imaging or MRI data of a fixed human brain hemisphere.Use 3D gradient magnetic fields to magnetize the tissue and collect signals at multiple time points or echoes to reduce background noise and artifacts and enhance the signals.Generate phase maps for all echoes, ensuring the removal of...
12:15
In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
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Cited by 7 •
2016
Here we present a novel Ca2+-imaging approach using a bioluminescent reporter. This approach uses a fused construct GFP-aequorin which binds to Ca2+ and emits light, eliminating the need for light excitation. Significantly this method permits long continuous imaging, access to deep brain structures and high temporal resolution.
FAQs
The measured signal determines which aspect of the brain becomes visible or quantifiable. Magnetic responses support structural or physiological measurements through magnetic resonance imaging, radiotracer distribution provides positron emission tomography data, and light enables optical imaging. Because these signals reflect different biological properties, researchers select or combine modalities according to whether they need anatomical, metabolic, or activity-related information.
No single imaging approach captures every feature of neural organization equally well. Differences in spatial and temporal resolution allow researchers to balance detailed localization against the timing of changing brain activity. Combining complementary measurements can therefore support more complete interpretations of connectivity, physiological responses, and relationships between neural events and behavior.
Measurements obtained while the brain remains within a living organism preserve relationships among neural systems, behavior, development, and disease. This context helps researchers connect observed structure or activity with ongoing biological processes rather than examining isolated tissue alone. Repeated measurements can also reveal changes over time, including progression or responses associated with treatment.
Selection depends on the information required and the signal that best represents it. Magnetic resonance imaging can address anatomical or physiological features, positron emission tomography can examine radiotracer distribution, and optical imaging can measure light-based signals. Researchers may use these approaches separately or comparatively to investigate structure, function, activity, metabolism, or connectivity.
Imaging allows neural measurements to be interpreted alongside observed behavior, helping researchers examine how brain systems support behavioral outcomes. Depending on the modality, the relevant data may describe anatomy, physiological function, activity, or metabolism. This linkage is useful for studying neural organization and for characterizing how brain processes change during development, disease, or treatment.
These methods allow researchers to characterize brain changes across developmental stages and disease states while preserving the living system being studied. Structural and physiological measurements can be compared across time or conditions to identify altered patterns. Such comparisons support investigations of neural organization, disease-related changes, and treatment-associated responses within neuroscience research.
Because measurements can be collected from a living organism over time, imaging provides a way to compare brain structure or physiology before and after treatment-related changes. The resulting patterns may show whether connectivity, metabolism, activity, or another measured feature has changed. This longitudinal perspective helps researchers evaluate treatment responses in relation to neural and behavioral outcomes.