Intracellular Injection
Intracellular Injection - Related Videos
07:18
Intracellular Refolding Assay
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Cited by 9 •
2012
In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.
11:18
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
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Cited by 234 •
2012
Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.
09:51
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
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Cited by 4 •
2012
We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.
02:57
Assessing Intracellular Bacterial Persistence and Antibiotic Resistance in a Mouse Model
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2026
Source: Zhang, C., et al., Development and Assessment of Intracellular Infection Models for Staphylococcus aureus. J. Vis. Exp. (2025)The video demonstrates the isolation of kidneys containing Staphylococcus aureus-infected macrophages, followed by tissue homogenization to release intracellular bacteria. It quantifies bacterial persistence within macrophages in control and antibiotic-treated groups by counting colony-forming units.
02:36
In Vivo Recording of Intracellular Signals from a Single Neuron
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2025
This video demonstrates the recording of electrical signals from single brain neurons using a fine-tipped glass micropipette and a specialized setup. The micropipette is carefully inserted into the rat's brain and is buzzed to penetrate the neuron to record internal neuronal electrical signals.
FAQs
Delivery is regulated by applying controlled pressure or electrical pulses through a fine glass micropipette after it has entered the cell. These inputs release a defined amount of material while the neuron remains under microscopic observation. Regulation matters because the technique is intended to target an individual living cell and connect the introduced substance with a subsequent cellular response.
Spatial precision comes from combining a microscale pipette with direct visual monitoring of the cell. This arrangement allows investigators to select a particular neuron rather than treating a broader population, then introduce a chosen substance into that cell. In neuroscience, that cell-level targeting supports relationships between a manipulated neuron’s contents, morphology, connectivity, or physiology.
Different cargoes support different readouts. Fluorescent dyes can make cellular structures visible, whereas tracers help investigate neuronal connectivity. Nucleic acids can be used when the goal concerns gene function, and pharmacological agents enable targeted manipulation of cellular physiology. Thus, the injected material links the intervention to the aspect of neuronal biology being examined.
Researchers first maintain the neuron under microscopic observation, then use a fine glass micropipette to penetrate the membrane. A controlled pressure or electrical pulse releases the selected substance into the cell. They can then examine the resulting label, tracer pattern, gene-related effect, or physiological response, depending on the material introduced and the study objective.
Neuroscientists choose it when an experiment requires a defined intervention in an individual neuron rather than an unspecified cellular population. Applications include labeling neuronal morphology, tracing connectivity, introducing nucleic acids to study gene function, and delivering pharmacological agents to examine cellular physiology. Its value lies in pairing precise cellular targeting with direct observation of outcomes.
By linking a targeted intervention to an observed neuronal response, the method provides a bridge between cellular events and larger questions about neural circuits and brain function. A labeled neuron can be examined for structure or connections, while a delivered nucleic acid or pharmacological agent can support studies of gene function or physiology. The same precision therefore serves complementary neuroscience investigations.