<em>In Vivo</em> Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Arianna R. Lark; Toshihiro Kitamoto; Jean-Ren&#233; Martin

doi:10.3791/53705

Journal

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Neuroscience

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In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

<em>In Vivo</em> Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

12,299 Views

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12:15 min

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January 08, 2016

DOI:

10.3791/53705-v

DOI:

10.3791/53705-v

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12:15 min

January 08, 2016

•

12273 Views

Arianna R. Lark^1,2, Toshihiro Kitamoto^2,3, Jean-René Martin¹

¹Equipe: Imagerie Cérébrale Fonctionnelle et Comportements (ICFC), Institut des Neurosciences Paris-Saclay (Nero-PSI),UMR-9197, CNRS/Université Paris Sud ²Interdisciplinary Program in Neuroscience, Graduate College,University of Iowa ³Department of Anesthesia, Carver College of Medicine,University of Iowa

Summary

Here we present a novel Ca²⁺-imaging approach using a bioluminescent reporter. This approach uses a fused construct GFP-aequorin which binds to Ca²⁺ and emits light, eliminating the need for light excitation. Significantly this method permits long continuous imaging, access to deep brain structures and high temporal resolution.