Microglial Depletion
Microglial Depletion - Related Videos
06:17
Depletion of Specific Cell Populations by Complement Depletion
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Cited by 15 •
2010
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
08:50
Depletion and Reconstitution of Macrophages in Mice
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Cited by 115 •
2012
Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.
08:10
Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester
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Cited by 11 •
2015
Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.
02:42
In Vivo Two-Photon Imaging of Microglial Dynamics in the Mouse Hippocampus
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2025
Source: Kamei, R., et. al., In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus. J. Vis. Exp. (2022)The video demonstrates the setup and imaging process for in vivo two-photon microscopy of microglial dynamics in the hippocampal CA1 (Cornu ammonis 1) and dentate gyrus, ensuring optimal optical alignment and structural integrity assessment.
11:07
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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Cited by 21 •
2014
In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.
FAQs
CSF1R inhibition reduces microglial survival signaling rather than merely changing an inflammatory state. Because CSF1R is described as a pathway required by microglia for survival, blocking it can produce substantial loss of these cells. This mechanism gives researchers a way to ask which neural or disease-related changes depend on microglial presence.
Targeted genetic approaches provide a complementary route to CSF1R-based depletion. The two strategies differ in how microglial reduction is produced, allowing experimental designs to examine whether an observed result is consistent across depletion methods. Such comparisons can strengthen interpretation, although the specific genetic targets and procedures depend on the experimental system.
Stopping the depletion treatment can create a recovery phase in which microglia repopulate the brain from surviving precursor cells. Comparing the depleted period with this repopulated state helps separate effects associated with microglial absence from changes that persist after their return. This comparison is informative for identifying reversible and longer-lasting consequences.
Depletion experiments require careful interpretation because removing microglia may cause changes that are consequences of the experimental manipulation, rather than evidence of a normal microglial function. Including repopulated brains helps reveal whether findings track the absence of microglia or remain after their return. This distinction matters when linking cellular changes to disease mechanisms.
A basic workflow begins by applying either CSF1R inhibition or a targeted genetic approach, followed by examination of the depleted brain state. If the treatment is removed, researchers can evaluate repopulation from surviving precursor cells. Comparing depleted and repopulated conditions organizes the experiment around microglial absence and return, helping identify changes associated with each state.
Microglial Depletion can help test microglial contributions to neuroinflammation, synaptic remodeling, neurodegeneration, and neural injury. Researchers can compare these processes when microglia are substantially reduced and after repopulation occurs. The resulting contrasts help distinguish changes associated with microglial activity from broader effects produced by removing the cells during the experiment.