Neuronal Activation

Neuronal activation is the process by which a neuron changes from a relatively inactive state to an electrically or chemically responsive state, forming the basis of communication in the nervous system. When excitatory inputs shift the membrane potential toward threshold, voltage-gated ion channels open, generating an action potential that travels along the axon and can trigger neurotransmitter release at synapses; inhibitory inputs reduce this likelihood. In psychology, studying neuronal activation helps link brain activity with perception, learning, emotion, and behavior through methods such as electrophysiology and functional neuroimaging. These measures clarify how coordinated neural circuits support cognition and how altered activation may accompany neurological or mental health conditions.

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JoVE EoE - Neurophysiology

Recording Electrophysiological Activity of the Neuronal Network in a Neuron-Astrocyte Co-culture

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2025

This video demonstrates establishing a neuron-astrocyte co-culture for recording neuronal network activity. Upon establishing the co-culture of motor neurons and astrocytes on a multi-electrode array (MEA) plate, the synchronous rhythmic electrical activity of the neuronal network was recorded extracellularly using the MEA.

Chemogenetic Silencing of Neuronal Activity in a Neonatal Mouse

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2025

Place an anesthetized neonatal pup on a heating pad.Disinfect the scalp with ethanol.Fill a syringe with a viral solution containing adeno-associated viruses carrying the gene for a drug-activated inhibitory receptor and a tracking dye.Align the syringe tip with the pup’s head and slowly inject the virus into the brain for even distribution.Allow the pup to recover.During incubation, viral particles enter neurons and release their genetic material into the nucleus.It leads to inhibitory...

Inhibition of Neuronal Activity and Its Detection Using Electrophysiology in a Rat

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2025

Source: Lai, J., et al. Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain. J. Vis. Exp. (2015)This video demonstrates a microinjection-based technique for simultaneous neuronal recording and inhibition in the rat superior colliculus. The procedure involves stereotaxic targeting, skull drilling, and the insertion of an injectrode, which delivers an inhibitory neurotransmitter while recording neuronal activity. Light stimulation evokes...

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

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Cited by 6 •

2013

Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.

Triggering a Closed-Loop Stimulation by Neuron Spiking Activity

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2025

This video demonstrates the operation of closed-loop stimulation in a rat with a surgically implanted device to record neural activity. Neuronal signals are first recorded and analyzed. Template waveforms are then uploaded, and parameters are defined to trigger closed-loop stimulation.

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