Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Mandy Stubbendorff; Xiaoqin Hua; Tobias Deuse; Ziad Ali; Hermann Reichenspurner; Lars Maegdefessel; Robert C. Robbins; Sonja Schrepfer

doi:10.3791/51459

Склонение Myointimal гиперплазия против атеросклероза у мышей: Введение двух допустимых моделей

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JoVE Journal Medicine

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Склонение Myointimal гиперплазия против атеросклероза у мышей: Введение двух допустимых моделей

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12,629 Views

08:34 min

May 14, 2014

DOI: 10.3791/51459-v

Mandy Stubbendorff*^1,2, Xiaoqin Hua*^1,2, Tobias Deuse^1,2,3, Ziad Ali^4,5, Hermann Reichenspurner^2,3, Lars Maegdefessel⁶, Robert C. Robbins⁷, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, Cardiovascular Research Center,University Hospital Hamburg, ²Cardiovascular Research Center (CVRC) and DZHK University Hamburg, ³Department of Cardiovascular Surgery,University Heart Center Hamburg, ⁴Center for Interventional Vascular Therapy, Division of Cardiology,Columbia University, ⁵Cardiovascular Research Foundation, New York, ⁶Karolinska Institute, Stockholm, ⁷Department of Cardiothoracic Surgery,Stanford University School of Medicine, Falk Cardiovascular Research Center

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Overview

This video presents two models of intimal plaque development in murine arteries, focusing on the mechanisms of myointimal hyperplasia and atherosclerosis.

Key Study Components

Area of Science

Cardiovascular biology
Vascular pathology
Animal models

Background

Intimal plaque development is crucial for understanding cardiovascular diseases.
Myointimal hyperplasia and atherosclerosis are two distinct processes leading to arterial narrowing.
Animal models are essential for studying these mechanisms.
This study utilizes murine models to investigate these conditions.

Purpose of Study

To introduce two valid animal models for studying aortic narrowing.
To compare the mechanisms of myointimal hyperplasia and atherosclerosis.
To enhance understanding of plaque development in arteries.

Methods Used

Balloon catheterization of the aorta to induce myointimal hyperplasia.
Dietary manipulation of transgenic APOE null mice to create atherosclerotic plaques.
Exposing and clamping the infrarenal abdominal aorta for surgical intervention.
Denuding the endothelium using an inflated catheter during the procedure.

Main Results

Successful induction of myointimal hyperplasia through balloon catheterization.
Formation of atherosclerotic plaques in transgenic APOE null mice via dietary changes.
Demonstration of distinct mechanisms leading to arterial narrowing.
Visual representation of plaque development in murine arteries.

Conclusions

Both models effectively demonstrate different pathways of intimal plaque development.
Understanding these mechanisms can inform therapeutic strategies for cardiovascular diseases.
Future studies may build on these models to explore further implications in vascular health.

Frequently Asked Questions

What is myointimal hyperplasia?

Myointimal hyperplasia is the proliferation of smooth muscle cells in the intima of blood vessels, often leading to narrowing.

How does atherosclerosis differ from myointimal hyperplasia?

Atherosclerosis involves the accumulation of lipids and inflammatory cells in the arterial wall, while myointimal hyperplasia is primarily a smooth muscle cell response.

Why are animal models used in this research?

Animal models provide a controlled environment to study complex biological processes and disease mechanisms that cannot be easily replicated in humans.

What role do transgenic mice play in this study?

Transgenic mice, such as APOE null mice, are used to study genetic influences on atherosclerosis and to model human disease more accurately.

What surgical techniques are employed in the balloon catheterization model?

The technique involves exposing the aorta, clamping it, and using a balloon catheter to denude the endothelium, simulating injury.

What are the implications of this research?

The findings can lead to better understanding and treatment of cardiovascular diseases by elucidating the mechanisms of plaque formation.

Это видео показывает две модели развития бляшек интимы в мышиных артерий и подчеркивает различия в myointimal гиперплазии и атеросклероза.

Общая цель данного эксперимента состоит в том, чтобы представить две валидные животные модели, приводящие к сужению аорты с помощью различных механизмов. Первой моделью является баллонная катетеризация аорты для создания развития миоинтимальной гиперплазии. Сначала обнажается и зажимается инфраренальная брюшная аорта.

Затем аорта вскрывается с помощью поперечного разреза и вводится баллонный катетер. Затем эндотелий аорты оголяется с помощью надутого катетера, и операция завершается. Вторая модель представляет собой диетические манипуляции с трансгенными мышами APOE null для создания атеросклеротических бляшек.

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