Fluorescent Antibody Staining in Cells

Fluorescent antibody staining uses a fluorescence microscope to make cells and microbes visible in bright colors against a dark background. The microscope works with fluorochromes, which are fluorescent dyes that absorb energy from a light source and then give off visible light. Some fluorochromes are natural, such as chloro...

In immunofluorescence microscopy, fluorophore labeled antibodies emit fluorescence upon binding a specific target or an antigen.

Immunofluorescence microscopy uses light to excite electrons of the fluorophore to a higher energy state. As they return to the ground state, the electrons release a longer wavelength of light.

This emiss...