Surface-Only Imaging with TIRF Microscopy

Total internal reflection fluorescence microscopy, or TIRF, is a microscope method used to view fluorophores near a solid surface with a higher refractive index, such as a glass coverslip. It is useful when scientists want to study signals close to the cell surface.

TIRF works when light from a m...

Total internal reflection fluorescence microscopy, or TIRF, selectively images fluorescent molecules in a sample close to a solid surface with a higher refractive index, such as a glass coverslip.

The excitation laser beam hits the coverslip at a critical angle, resulting in total internal reflection of the beam.

The reflected ligh...