Antibody Staining in Cells and Tissues

Immunocytochemistry (ICC) and immunohistochemistry (IHC) use antibodies to detect specific proteins or antigens in samples. ICC focuses on individual cells, such as blood cells and stem cells. IHC is used for tissue samples. These methods were first published by Albert Coons in 1941, when they were used to detect pneumococca...

Immunocytochemistry or ICC and immunohistochemistry or IHC are staining techniques that use enzyme-linked antibodies to visualize and quantify specific proteins or antigens in cultured cells or tissue sections.

In ICC, a single layer of targeted cells is grown on a glass coverslip. The cells are fixed with a cross-linking agent to prevent...