All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
- Surgical procedure
NOTE: The chronic cisterna magna (CM) cannulation allows the animals to recover from the surgical procedure. CM injections are made the day following cannula implantation and, importantly, can be performed in either anesthetized or awake animals. Since this is a recovery surgery, procedures should be carried out under sterile conditions.- Weigh the mouse (C57BL/6JRj, both sexes, 8 weeks) and anesthetize it with a mixture of ketamine and xyl....