Cannula Implantation into the Cisterna Magna in a Mouse Model

Abstract

Source: Xavier, A. L. R., et al. Cannula Implantation into the Cisterna Magna of Rodents. J. Vis. Exp. (2018)

This video demonstrates the procedure for implanting a cannula into the cisterna magna of an anesthetized mouse. The technique involves guiding a needle-mounted cannula through the dura into the inverted triangular cisterna magna and securing it for targeted delivery into the cerebrospinal fluid.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgical procedure
   NOTE: The chronic cisterna magna (CM) cannulation allows the animals to recover from the surgical procedure. CM injections are made the day following cannula implantation and, importantly, can be performed in either anesthetized or awake animals. Since this is a recovery surgery, procedures should be carried out under sterile conditions.
   1. Weigh the mouse (C57BL/6JRj, both sexes, 8 weeks) and anesthetize it with a mixture of ketamine and xyl....

Results

Figure 1: Injection of tracers into the cisterna magna. (A) Schematic overview of the m.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
SOPIRA carpule 30G 0.3 x 12 mm	Kulzer	AA001
Chlorhexidine 0.5% (chlorhexidine digluconate)	Meda AS		http://www.meda.dk/behandlingsomrader/desinfektion/desinfektion-af-hud/klorhexidin-sprit-medic-05/
Alcohol swab 70% isopropyl alcohol 30 x 60 mm	Vitrex Medical A/S	520213
Viskoese Oejendraeber Ophtha	Ophtha	145250
Wooden applicator, double cotton bud (Ø approximately, 4 - 5 mm, length approximately, 12 mm)	Heinz Herenz	1032018
Eye spears	Medicom	A18005
Kimtech Science precision wipes tissue wipers	Kimberly Clark Professional	5511
Loctite super glue precision 5 g	Loctite		http://www.loctite-consumer.dk/da/produkter/superglue-liquid.html
Insta-Set CA accelerator	Bob Smith Industries	BSI-152
Dental cement powder	A-M Systems	525000
Phosphate-buffered saline (PBS; 0.01 M; pH 7.4)	Sigma Aldrich	P3813
Ovalbumin, Alexa Fluor 647 conjugate	Thermo Fisher Scientific	O34784
DAPI (4′,6-diamidino-2-phenylindole) solution (1 mg/mL)	Thermo Fisher Scientific	62248
Dextran, fluorescein, 3000 MW, anionic	Thermo Fisher Scientific	D3305
Ketaminol Vet 100 mg/mL (ketamine)	Intervet International BV	511519
Rompin Vet 20 mg/mL (xylazine)	KVP Pharma + Veterinär Produkte GmbH	148999
Xylocain 20 mg/mL (lidocain)	AstraZeneca	158543
Marcain 2.5 mg/mL (bupivacain)	AstraZeneca	123918
Bupaq Vet 0.3 mg/mL (buprenorphine)	Richter Pharma AG	185159